Brand  (β version)

PDB ID: 5FYQ

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Ligands
Code Name Style Show Link
SO4 Sulfate ion PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
Code Name Show
FAK N~6~-(trifluoroacetyl)-L-lysine
Glycosylation
Code Name Emphasize
Modification
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Structure summary

Code : 5FYQ   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : Sirt2 in complex with a 13-mer trifluoroacetylated Ran peptide
Release Data : 2016-05-25
Compound :
mol_id molecule chains synonym
1 NAD-DEPENDENT PROTEIN DEACETYLASE SIRTUIN-2 A,B REGULATORY PROTEIN SIR2 HOMOLOG 2, SIR2-LIKE PROTEIN 2
ec: 3.5.1.-
fragment: 50-356
mol_id molecule chains
2 RAN AA 31-43 C,D
fragment: PART OF SWITCH I, RESIDUES 31-43
other_details: TRIFLUOROACETYLATED AT K37
Source :
mol_id organism_scientific organism_common expression_system
1 HOMO SAPIENS  (taxid:9606) HUMAN ESCHERICHIA COLI  (taxid:469008)
gene: SIRT2, SIR2L, SIR2L2
expression_system_strain: BL21(DE3)
expression_system_plasmid: PGEX
mol_id organism_scientific organism_common
2 HOMO SAPIENS  (taxid:9606) HUMAN
synthetic: yes
Authors : Knyphausen, P., de Boor, S., Scislowski, L., Extra, A., Baldus, L., Schacherl, M., Baumann, U., Neundorf, I., Lammers, M.
Keywords : HYDROLASE, SIRTUIN, KDAC, LYSINE-DEACETYLASE, LYSINE-ACETYLATION, GENETIC-CODE EXPANSION
Exp. method : X-RAY DIFFRACTION ( 3.00 Å )
Citation :

Insights Into Lysine-Deacetylation of Natively Folded Substrate Proteins by Sirtuins.

Knyphausen, P.,De Boor, S.,Kuhlmann, N.  et al.
(2016)  J.Biol.Chem.  291 : 14677

PubMed: 27226597
DOI: 10.1074/JBC.M116.726307

Reaction

Chain : A, B
UniProt : Q8IXJ6 (SIR2_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
H2O + N(6)-acetyl-L-lysyl-[protein] + NAD(+) = 2''-O-acetyl- ADP-D-ribose + L-lysyl-[protein] + nicotinamide 2.3.1.286 PROSITE-ProRule:PRU00236, PubMed:11483616, PubMed:11812793, PubMed:16648462, PubMed:18722353, PubMed:24177535, PubMed:24389023, PubMed:24940000, PubMed:25672491
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H2O + N(6)-tetradecanoyl-L-lysyl-[protein] + NAD(+) = 2''-O- tetradecanoyl-ADP-D-ribose + L-lysyl-[protein] + nicotinamide - PubMed:25704306, PubMed:32103017
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H2O + N(6)-hexadecanoyl-L-lysyl-[protein] + NAD(+) = 2''-O- hexadecanoyl-ADP-D-ribose + L-lysyl-[protein] + nicotinamide - PubMed:29239724
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Chain : C, D
UniProt : P62826 (RAN_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
GTP + H2O = GDP + H(+) + phosphate - PubMed:26272610, PubMed:8636225
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