Brand  (β version)

PDB ID: 5FWG

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Ligands
Code Name Style Show Link
GPR (9r,10r)-9-(S-glutathionyl)-10-hydroxy-9,10-dihydrophenanthrene PoSSuM
Non-standard Residues
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FTR Fluorotryptophane
Glycosylation
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Structure summary

Code : 5FWG   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : TETRA-(5-FLUOROTRYPTOPHANYL)-GLUTATHIONE TRANSFERASE
Release Data : 1999-01-27
Compound :
mol_id molecule chains synonym
1 TETRA-(5-FLUOROTRYPTOPHANYL)-GLUTATHIONE TRANSFERASE MU CLASS A,B RAT MU CLASS GST, RAT M1-1 GST
ec: 2.5.1.18
mutation: 4 TRYPTOPHANS MUTATED TO 5-FLUOROTRYPTOPHANS
Source :
mol_id organism_scientific organism_common expression_system
1 Rattus norvegicus  (taxid:10116) Norway rat Escherichia coli BL21(DE3)  (taxid:469008)
cell_line: BL21
organ: LIVER
gene: CDNA INSERT OF 3-3 (M1-1) ENZY
expression_system_strain: BL21 (DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: PSW1GST33
expression_system_gene: CDNA INSERT OF 3-3 (M1-1) ENZYME
Authors : Parsons, J.F., Xiao, G., Armstrong, R.N., Gilliland, G.L.
Keywords : GLUTATHIONE TRANSFERASE, UNNATURAL AMINO ACID, 5-FLUOROTRYPTOPHAN, THREE-DIMENSIONAL STRUCTURE, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 2.0 Å )
Citation :

Enzymes harboring unnatural amino acids: mechanistic and structural analysis of the enhanced catalytic activity of a glutathione transferase containing 5-fluorotryptophan.

Parsons, J.F.,Xiao, G.,Gilliland, G.L.  et al.
(1998)  Biochemistry  37 : 6286 - 6294

PubMed: 9572843
DOI: 10.1021/bi980219e

Tyrosine 115 Participates Both in Chemical and Physical Steps of the Catalytic Mechanism of a Glutathione S-Transferase

Johnson, W.W.,Liu, S.,Ji, X.  et al.
(1993)  J.Biol.Chem.  268 : 11508

Contribution of Tyrosine 6 to the Catalytic Mechanism of Isoenzyme 3-3 of Glutathione S-Transferase

Liu, S.,Zhang, P.,Ji, X.  et al.
(1992)  J.Biol.Chem.  267 : 4296

The Three-Dimensional Structure of a Glutathione S-Transferase from the Mu Gene Class. Structural Analysis of the Binary Complex of Isoenzyme 3-3 and Glutathione at 2.2-A Resolution

Ji, X.,Zhang, P.,Armstrong, R.N.  et al.
(1992)  Biochemistry  31 : 10169

Reaction

Chain : A, B
UniProt : P04905 (GSTM1_RAT)
Reaction: EC: Evidence:
Physiological Direction:
RX + glutathione = an S-substituted glutathione + a halide anion + H(+) 2.5.1.18 PubMed:8110735, PubMed:8664265
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prostaglandin A2 + glutathione = prostaglandin A2-S-(R)- glutathione - UniProtKB:P09488
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prostaglandin J2 + glutathione = prostaglandin J2-S-(R)- glutathione - UniProtKB:P09488
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prostaglandin J2 + glutathione = prostaglandin J2-S-(S)- glutathione - UniProtKB:P09488
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prostaglandin A2 + glutathione = prostaglandin A2-S-(S)- glutathione - UniProtKB:P09488
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11(S)-hydroxy-14(S),15(S)-epoxy-(5Z,8Z,12E)-eicosatrienoate + glutathione = (11S,15S)-dihydroxy-14(R)-S-glutathionyl-(5Z,8Z,12E)- eicosatrienoate - UniProtKB:P09488
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