Brand  (β version)

PDB ID: 5ET4

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Ligands
Code Name Style Show Link
C3P Cytidine-3'-monophosphate PoSSuM
MPD (4s)-2-methyl-2,4-pentanediol PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 5ET4   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : Structure of RNase A-K7H/R10H in complex with 3'-CMP
Release Data : 2016-11-30
Compound :
mol_id molecule chains synonym
1 Ribonuclease pancreatic A,B,C,D RNase 1,RNase A
ec: 3.1.27.5
mutation: K7H, R10H
Source :
mol_id organism_scientific organism_common expression_system
1 Bos taurus  (taxid:9913) Bovine Escherichia coli BL21  (taxid:511693)
gene: RNASE1, RNS1
expression_system_vector_type: PLASMID
expression_system_plasmid: pET11c
Authors : Blanco, J.A., Salazar, V.A., Moussaoui, M., Boix, E.
Keywords : RNase A, p2 subsite, exonuclease activity, hydrolase
Exp. method : X-RAY DIFFRACTION ( 2.10 Å )
Citation :

Characterization of an RNase with two catalytic centers. Human RNase6 catalytic and phosphate-binding site arrangement favors the endonuclease cleavage of polymeric substrates.

Prats-Ejarque, G.,Blanco, J.A.,Salazar, V.A.  et al.
(2019)  Biochim Biophys Acta Gen Subj  1863 : 105 - 117

PubMed: 30287244
DOI: 10.1016/j.bbagen.2018.09.021

Reaction

Chain : A, B, C, D
UniProt : P61823 (RNAS1_BOVIN)
Reaction: EC: Evidence:
Physiological Direction:
an [RNA] containing cytidine + H2O = an [RNA]-3'-cytidine-3'- phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA]. 4.6.1.18 -
-
an [RNA] containing uridine + H2O = an [RNA]-3'-uridine-3'- phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA]. 4.6.1.18 -
-