Brand  (β version)

PDB ID: 5ECN

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Ligands
Code Name Style Show Link
JAA {(1r,2r)-3-oxo-2-[(2z)-pent-2-en-1-yl]cyclopentyl}acetic acid PoSSuM
LEU Leucine PoSSuM
ATP Adenosine-5'-triphosphate PoSSuM
GSH Glutathione PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 5ECN   PDBj   RCSB PDB   PDBe
Header : LIGASE/TRANSFERASE
Title : Crystal Structure of FIN219-FIP1 complex with JA, Leu and ATP
Release Data : 2016-11-02
Compound :
mol_id molecule chains synonym
1 Jasmonic acid-amido synthetase JAR1 A,D Jasmonate-amino acid synthetase JAR1,Protein FAR-RED INSENSITIVE 219,Protein JASMONATE RESISTANT 1
ec: 6.3.2.-
mol_id molecule chains synonym
2 Glutathione S-transferase U20 B,C,E,F AtGSTU20,FIN219-interacting protein 1,GST class-tau member 20
ec: 2.5.1.18
Source :
mol_id organism_scientific organism_common expression_system
1 Arabidopsis thaliana  (taxid:3702) Mouse-ear cress Escherichia coli BL21(DE3)  (taxid:469008)
gene: JAR1, FIN219, At2g46370, F11C10.6
expression_system_strain: BL21(DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pGEX-4T-1
mol_id organism_scientific organism_common expression_system
2 Arabidopsis thaliana  (taxid:3702) Mouse-ear cress Escherichia coli  (taxid:562)
gene: GSTU20, FIP1, At1g78370, F3F9.11
expression_system_strain: BL21(DE3)pLysS
expression_system_vector_type: plasmid
expression_system_plasmid: pRSET-B
Authors : Chen, C.Y., Cheng, Y.S.
Keywords : Jasmonate-amido synthetase, Glutathione S-transferase, Ligase-Transferase complex
Exp. method : X-RAY DIFFRACTION ( 1.7200 Å )
Citation :

Structural basis of jasmonate-amido synthetase FIN219 in complex with glutathione S-transferase FIP1 during the JA signal regulation

Chen, C.Y.,Ho, S.S.,Kuo, T.Y.  et al.
(2017)  Proc. Natl. Acad. Sci. U.S.A.  114 : E1815 - E1824

PubMed: 28223489
DOI: 10.1073/pnas.1609980114

Structural basis for prereceptor modulation of plant hormones by GH3 proteins.

Westfall, C.S.,Zubieta, C.,Herrmann, J.  et al.
(2012)  Science  336 : 1708 - 1711

PubMed: 22628555
DOI: 10.1126/science.1221863

Reaction

Chain : A, D
UniProt : Q9SKE2 (JAR1_ARATH)
Reaction: EC: Evidence:
Physiological Direction:
a jasmonate + an L-alpha-amino acid + ATP = a jasmonyl-L-amino acid + AMP + diphosphate + H(+) 6.3.2.52 PubMed:18247047
-
(+)-7-isojasmonate + ATP + L-isoleucine = AMP + diphosphate + H(+) + L-isoleucine-(+)-7-isojasmonate - PubMed:15258265
left-to-right
Chain : B, C, E, F
UniProt : Q8L7C9 (GSTUK_ARATH)
Reaction: EC: Evidence:
Physiological Direction:
glutathione + RX = a halide anion + an S-substituted glutathione + H(+) 2.5.1.18 PubMed:17220357, PubMed:28223489
-