Het-PDB Navi2 (PDB: 5EAO)

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Ligands
Code	Name		Style	Show	Link
MG	Magnesium ion				PoSSuM

Non-standard Residues
Code	Name	Show
CVC	[(1~{R},5~{R},6~{R},8~{R})-6-(4-azanyl-2-oxidanylidene-pyrimidin-1-yl)-3,3-bis(oxidanyl)-2,4,7-trioxa-3$L^{4}-vanadabicyclo[3.3.0]octan-8-yl]methyl dihydrogen phosphate

Glycosylation
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Modification
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Download interaction data: 5EAO

Code :

5EAO PDBj RCSB PDB PDBe

Header :

RNA

Title :

Two active site divalent ion in the crystal structure of the hammerhead ribozyme bound to a transition state analog-Mg2+

Release Data :

2016-02-24

Compound :

mol_id	molecule	chains
1	RNA (48-MER)	A

mol_id	molecule	chains
2	RNA (5'-R(GPGPGPCPGPU(CVC)UPGPGPGPCPAPGPUPAPCPCPCPA)-3')	B

Source :

mol_id	organism_scientific	expression_system
1	Synthetic construct (taxid:32630)	Escherichia coli (taxid:562)
	expression_system_strain: DH5alpha expression_system_vector_type: plasmid expression_system_plasmid: pUC19

mol_id	organism_scientific
2	Synthetic construct (taxid:32630)
	synthetic: yes

Authors :

Mir, A., Golden, B.L.

Keywords :

ribozyme, hammerhead, RNA

Exp. method :

X-RAY DIFFRACTION ( 2.988 Å )

Citation :

Two Active Site Divalent Ions in the Crystal Structure of the Hammerhead Ribozyme Bound to a Transition State Analogue.

Mir, A.,Golden, B.L.
(2016) Biochemistry 55 : 633 - 636

Two Divalent Metal Ions and Conformational Changes Play Roles in the Hammerhead Ribozyme Cleavage Reaction.

Mir, A.,Chen, J.,Robinson, K. et al.
(2015) Biochemistry 54 : 6369 - 6381

PDB ID: 5EAO