Brand  (β version)

PDB ID: 5BW6

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Code Name Style Show Link
F6F 2-{[4-(trifluoromethoxy)benzoyl]amino}ethyl dihydrogen phosphate PoSSuM
NA Sodium ion PoSSuM
PLP Pyridoxal-5'-phosphate PoSSuM

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Structure summary

Code : 5BW6   PDBj   RCSB PDB   PDBe
Header : LYASE/LYASE INHIBITOR
Title : Tryptophan Synthase from Salmonella typhimurium in complex with a single molecule of 2-({[4-(trifluoromethoxy)phenyl]carbonyl}amino)ethyl dihydrogen phosphate (F6) in the alpha-site.
Release Data : 2016-02-10
Compound :
mol_id molecule chains
1 Tryptophan synthase alpha chain A
ec: 4.2.1.20
mol_id molecule chains
2 Tryptophan synthase beta chain B
ec: 4.2.1.20
Source :
mol_id organism_scientific expression_system
1 Salmonella enterica subsp. enterica serovar Typhimurium  (taxid:90371) Escherichia coli  (taxid:562)
gene: trpA
expression_system_strain: Cb149
expression_system_vector_type: plasmid
expression_system_plasmid: Derivative of Pbr322
mol_id organism_scientific expression_system
2 Salmonella enterica subsp. enterica serovar Typhimurium  (taxid:90371) Escherichia coli  (taxid:562)
gene: trpB
expression_system_strain: Cb149
expression_system_vector_type: plasmid
expression_system_plasmid: Derivative of Pbr322
Authors : Hilario, E., Caulkins, B.G., Young, R.P., Niks, D., Dunn, M.F., Mueller, L.J., Fan, L.
Keywords : carbon-oxygen lyase, tryptophan biosynthesis, Salmonella typhimurium, F6F, inhibitor, allosteric enzyme, aromatic amino acid biosynthesis, pyridoxal phosphate, TRANSFERASE, LYASE-LYASE INHIBITOR complex
Exp. method : X-RAY DIFFRACTION ( 1.8200 Å )
Citation :

Visualizing the tunnel in tryptophan synthase with crystallography: Insights into a selective filter for accommodating indole and rejecting water.

Hilario, E.,Caulkins, B.G.,Huang, Y.M.  et al.
(2016)  Biochim.Biophys.Acta  1864 : 268 - 279

PubMed: 26708480
DOI: 10.1016/j.bbapap.2015.12.006

Reaction

Chain : A
UniProt : P00929 (TRPA_SALTY)
Reaction: EC: Evidence:
Physiological Direction:
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D- glyceraldehyde 3-phosphate + L-tryptophan + H2O 4.2.1.20 HAMAP-Rule:MF_00131
-
Chain : B
UniProt : P0A2K1 (TRPB_SALTY)
Reaction: EC: Evidence:
Physiological Direction:
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D- glyceraldehyde 3-phosphate + L-tryptophan + H2O 4.2.1.20 -
-
Cofactor: Evidence: Note:
pyridoxal 5'-phosphate - -