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Ligands
Code Name Style Show Link
BGC Beta-D-glucopyranose
G2F 2-deoxy-2-fluoro-alpha-D-glucopyranose
: Polysaccharide
Non-standard Residues
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Glycosylation
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Code : 5A3H   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : 2-DEOXY-2-FLURO-B-D-CELLOBIOSYL/ENZYME INTERMEDIATE COMPLEX OF THE ENDOGLUCANASE CEL5A FROM BACILLUS AGARADHEARANS AT 1.8 ANGSTROMS RESOLUTION
Release Data : 1999-07-24
Compound :
mol_id molecule chains synonym
1 ENDOGLUCANASE A CELLULASE
ec: 3.2.1.4
fragment: CATALYTIC CORE DOMAIN ONLY
other_details: THIS IS A COMPLEX WITH 2-DEOXY-2-FLUOROCELLOBIOSE COVALENTLY LINKED TO THE ENZYMATIC NUCLEOPHILE, GLU 228
Source :
mol_id organism_scientific expression_system
1 Bacillus agaradhaerens  (taxid:76935) Bacillus subtilis  (taxid:1423)
strain: AC13 (NCIMB 40482)
expression_system_strain: PL2306
expression_system_vector_type: BACILLUS, CELLULASE NEGATIVE STRAIN
expression_system_vector: PMOL995
expression_system_plasmid: THERMAMYL-AMYLASE PROMOTER SYSTEM
Authors : Davies, G.J., Varrot, A., Dauter, M., Brzozowski, A.M., Schulein, M., Mackenzie, L., Withers, S.G.
Keywords : HYDROLASE, CELLULOSE DEGRADATION, ENDOGLUCANASE, GLYCOSIDE HYDROLASE FAMILY 5, MICHAELIS COMPLEX, SKEW-BOAT, DISTORTION
Exp. method : X-RAY DIFFRACTION ( 1.82 Å )
Citation :

Snapshots along an enzymatic reaction coordinate: analysis of a retaining beta-glycoside hydrolase.

Davies, G.J.,Mackenzie, L.,Varrot, A.  et al.
(1998)  Biochemistry  37 : 11707 - 11713

PubMed: 9718293
DOI: 10.1021/bi981315i

Structure of the Bacillus Agaradherans Family 5 Endoglucanase at 1.6 A and its Cellobiose Complex at 2.0 A Resolution

Davies, G.J.,Dauter, M.,Brzozowski, A.M.  et al.
(1998)  Biochemistry  37 : 1926

Chain : A
UniProt : O85465 (GUN5_SALAG)
Reaction: EC: Evidence:
Physiological Direction:
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans. 3.2.1.4 PubMed:9485319, PubMed:9718293
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