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Ligands
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Non-standard Residues
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CRO {2-[(1r,2r)-1-amino-2-hydroxypropyl]-4-(4-hydroxybenzylidene)-5-oxo-4,5-dihydro-1h-imidazol-1-yl}acetic acid
Glycosylation
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Code : 4XL5   PDBj   RCSB PDB   PDBe
Header : PROTEIN BINDING
Title : X-ray structure of bGFP-A / EGFP complex
Release Data : 2015-08-19
Compound :
mol_id molecule chains
1 Green fluorescent protein A
mol_id molecule chains
2 bGFP-A C
Source :
mol_id organism_scientific organism_common expression_system
1 Aequorea victoria  (taxid:6100) Jellyfish Escherichia coli  (taxid:562)
gene: GFP
expression_system_vector_type: plasmid
expression_system_plasmid: PQE81L
mol_id organism_scientific expression_system
2 Synthetic construct  (taxid:32630) Escherichia coli  (taxid:562)
expression_system_vector_type: plasmid
expression_system_plasmid: PQE81L
Authors : Chevrel, A., Urvoas, A., Li de la Sierra-Gallay, I., Van Tilbeurgh, H., Minard, P., Valerio-Lepiniec, M.
Keywords : AlphaRep Scaffold, Complex, EGFP, PROTEIN ENGINEERING, HEAT-LIKE REPEAT, PROTEIN BINDING
Exp. method : X-RAY DIFFRACTION ( 2.00 Å )
Citation :

Specific GFP-binding artificial proteins ( alpha Rep): a new tool for in vitro to live cell applications.

Chevrel, A.,Urvoas, A.,de la Sierra-Gallay, I.L.  et al.
(2015)  Biosci.Rep.  35

PubMed: 26182430
DOI: 10.1042/BSR20150080

Chain : A
UniProt : P42212 (GFP_AEQVI)
Reaction : -