Brand  (β version)

PDB ID: 4RSP

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Ligands
Code Name Style Show Link
Non-standard Residues
Code Name Show
CSO S-hydroxycysteine
Glycosylation
Code Name Emphasize
Modification
Code Name Show
BOC Tert-butyl hydrogen carbonate
CEV Ethyl (4r)-4-amino-5-[(3s)-2-oxopyrrolidin-3-yl]pentanoate

Structure summary

Code : 4RSP   PDBj   RCSB PDB   PDBe
Header : Hydrolase/hydrolase Inhibitor
Title : X-ray structure of MERS-CoV nsp5 protease bound with a designed inhibitor
Release Data : 2015-06-17
Compound :
mol_id molecule chains
1 Orf1a protein A
mol_id molecule chains
2 Peptide inhibitor B
Source :
mol_id organism_scientific expression_system
1 Middle East respiratory syndrome coronavirus  (taxid:1335626) Escherichia coli  (taxid:469008)
gene: orf1a
expression_system_strain: BL21-DE3
expression_system_vector_type: plasmid
expression_system_plasmid: pET-11a
mol_id organism_scientific
2
synthetic: yes
other_details: The peptide inhibitor was chemically synthesized in the lab.
Authors : Tomar, S., Mesecar, A.D.
Keywords : protease, Hydrolase-hydrolase Inhibitor complex
Exp. method : X-RAY DIFFRACTION ( 1.620 Å )
Citation :

Ligand-induced Dimerization of Middle East Respiratory Syndrome (MERS) Coronavirus nsp5 Protease (3CLpro): IMPLICATIONS FOR nsp5 REGULATION AND THE DEVELOPMENT OF ANTIVIRALS.

Tomar, S.,Johnston, M.L.,St John, S.E.  et al.
(2015)  J.Biol.Chem.  290 : 19403 - 19422

PubMed: 26055715
DOI: 10.1074/jbc.M115.651463

Reaction

Chain : A
UniProt : V9TU12 (V9TU12_MERS)
Reaction: EC: Evidence:
Physiological Direction:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76- residue protein attached to proteins as an intracellular targeting signal). 3.4.19.12 ARBA:ARBA00000707
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