Brand  (β version)

PDB ID: 4OJX

Hetero Atom Contents

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain:

Ligands
Code Name Link
G Guanosine-5'-monophosphate PoSSuM
ZN Zinc ion PoSSuM
MPD (4s)-2-methyl-2,4-pentanediol PoSSuM

Structure summary

Code : 4OJX
Header : HYDROLASE
Title : crystal structure of yeast phosphodiesterase-1 in complex with GMP
Release Data : 2014-12-03
Compound :
mol_id molecule chains synonym
1 3',5'-cyclic-nucleotide phosphodiesterase 1 A PDEase 1, 3':5'-CNP, Low-affinity cAMP phosphodiesterase
ec: 3.1.4.17
Source :
mol_id organism_scientific organism_common expression_system
1 Saccharomyces cerevisiae  (taxid:559292) Baker's yeast Escherichia coli  (taxid:511693)
strain: ATCC 204508 / S288c
gene: PDE1, YGL248W, NRB369
expression_system_strain: BL21
expression_system_vector_type: plasmid
expression_system_plasmid: pET28a
Authors : Tian, Y., Cui, W., Huang, M., Robinson, H., Wan, Y., Wang, Y., Ke, H.
Keywords : phosphodiesterase, cGMP and cAMP, yeast PDE, dual specificity, HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 1.31 Å )
Citation :

Dual specificity and novel structural folding of yeast phosphodiesterase-1 for hydrolysis of second messengers cyclic adenosine and guanosine 3',5'-monophosphate.

Tian, Y.,Cui, W.,Huang, M.  et al.
(2014)  Biochemistry  53 : 4938 - 4945

PubMed: 25050706
DOI: 10.1021/bi500406h

Reaction

Chain : A
UniProt : P22434 (PDE1_YEAST)
Reaction : Nucleoside 3',5'-cyclic phosphate + H(2)O = nucleoside 5'-phosphate.