Brand  (β version)

PDB ID: 4MIK

Hetero Atom Contents

  The number of atoms exceeds 100,000.
  So, it can not be displayed here.

Select unit:

Select hetatm:   

close
information
centroid:
interaction residue:

Select chain:   Sequence  

Data format:   

Color scheme of protein:

Ligands
Code Name Style Show Link
JIL 9-{5-S-[2-({[(3s)-7-nitro-1,2,3,4-tetrahydroisoquinolin-3-yl]methyl}amino)ethyl]-5-thio-alpha-L-lyxofuranosyl}-9h-purin-6-amine PoSSuM
TRS 2-amino-2-hydroxymethyl-propane-1,3-diol PoSSuM
Non-standard Residues
Code Name Show
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 4MIK   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE/TRANSFERASE INHIBITOR
Title : Crystal Structure of hPNMT in Complex with bisubstrate inhibitor (2R,3R,4S,5S)-2-(6-amino-9H-purin-9-yl)-5-(((2-(((7-nitro-1,2,3,4-tetrahydroisoquinolin-3-yl)methyl)amino)ethyl)thio)methyl)tetrahydrofuran-3,4-diol
Release Data : 2014-09-10
Compound :
mol_id molecule chains synonym
1 Phenylethanolamine N-methyltransferase A,B PNMTase, Noradrenaline N-methyltransferase
ec: 2.1.1.28
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:562)
gene: PNMT, PENT
expression_system_strain: BL21(DE3)pLysE
expression_system_vector_type: PLASMID
expression_system_plasmid: pET17PNMT-His
Authors : Bart, A.G., Scott, E.E.
Keywords : methyltransferase, adrenaline synthesis, TRANSFERASE-TRANSFERASE INHIBITOR complex
Exp. method : X-RAY DIFFRACTION ( 1.9506 Å )
Citation :

To be published

Bart, A.G.,Scott, E.E.
To be Published 

Reaction

Chain : A, B
UniProt : P11086 (PNMT_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
phenylethanolamine + S-adenosyl-L-methionine = H(+) + N- methylphenylethanolamine + S-adenosyl-L-homocysteine 2.1.1.28 PubMed:16363801, PubMed:8812853
left-to-right PubMed:16363801
(R)-noradrenaline + S-adenosyl-L-methionine = (R)-adrenaline + H(+) + S-adenosyl-L-homocysteine 2.1.1.28 PubMed:20496117
left-to-right
(R)-normetanephrine + S-adenosyl-L-methionine = (R)- metanephrine + H(+) + S-adenosyl-L-homocysteine - UniProtKB:P10938
left-to-right
(R)-octopamine + S-adenosyl-L-methionine = (R)-synephrine + H(+) + S-adenosyl-L-homocysteine - PubMed:16277617, PubMed:16363801
left-to-right PubMed:16363801