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Code Name Link
FS5 Iron/sulfur penta-sulfide connected clusters
Code : 4JC0
Title : Crystal structure of Thermotoga maritima holo RimO in complex with pentasulfide, Northeast Structural Genomics Consortium Target VR77
Release Data : 2013-04-10
Compound :
mol_id molecule chains synonym
1 Ribosomal protein S12 methylthiotransferase RimO A,B S12 MTTase, S12 methylthiotransferase, Ribosome maturation factor RimO
ec: 2.-.-.-
Source :
mol_id organism_scientific expression_system
1 Thermotoga maritima  (taxid:2336) Escherichia coli  (taxid:469008)
strain: ATCC 43589
gene: rimO, TM1862, TM_1862
expression_system_strain: BL21(DE3)+Magic
expression_system_vector_type: plasmid
expression_system_plasmid: pET21
Authors : Forouhar, F., Hussain, M., Seetharaman, J., Fang, Y., Chen, C.X., Cunningham, K., Conover, K., Ma, L.-C., Xiao, R., Acton, T.B., Montelione, G.T., Tong, L., Hunt, J.F., Northeast Structural Genomics Consortium (NESG)
Keywords : Structural Genomics, PSI-Biology, Northeast Structural Genomics Consortium, NESG, three-domained enzyme, alpha-beta N-terminal domain, Semi-TIM barrel Radical-SAM domain, beta barrel C-terminal TRAM domain, ribosomal protein S12, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 3.30 Å )
Citation :

Two Fe-S clusters catalyze sulfur insertion by radical-SAM methylthiotransferases.

Forouhar, F.,Arragain, S.,Atta, M.  et al.
(2013)  Nat.Chem.Biol.  9 : 333 - 338

PubMed: 23542644
DOI: 10.1038/nchembio.1229

Chain : A, B
UniProt : Q9X2H6 (RIMO_THEMA)
Reaction : L-aspartate-[ribosomal protein S12] + sulfur- (sulfur carrier) + 2 S-adenosyl-L-methionine = 3-methylthio-L- aspartate-[ribosomal protein S12] + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine.