Brand  (β version)

PDB ID: 4II9

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Ligands
Code Name Style Show Link
GOL Glycerol PoSSuM
UDP Uridine-5'-diphosphate PoSSuM
MUB N-acetyl-alpha-muramic acid PoSSuM
Non-standard Residues
Code Name Show
A9Z 2'-deoxy-2'-(4-ethyl-1h-1,2,3-triazol-1-yl)adenosine 5'-(dihydrogen phosphate)
DAL D-alanine
FGA Gamma-D-glutamic acid
Glycosylation
Code Name Emphasize
Modification
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Structure summary

Code : 4II9   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE/PEPTIDE/RNA
Title : Crystal structure of Weissella viridescens FemXVv non-ribosomal amino acid transferase in complex with a peptidyl-RNA conjugate
Release Data : 2013-07-03
Compound :
mol_id molecule chains
1 FemX A
ec: 2.3.2.10
mol_id molecule chains
2 5-mer peptide B
mol_id molecule chains
3 RNA (5'-R(P*CP*CP*(A9Z))-3') C
Source :
mol_id organism_scientific expression_system
1 Weissella viridescens  (taxid:1629) Escherichia coli  (taxid:562)
strain: CIP102810T
gene: femX
expression_system_strain: TOP10
expression_system_vector_type: PLASMID
expression_system_plasmid: pTrc-His60
mol_id organism_scientific
2
synthetic: yes
other_details: synthetic peptide
mol_id organism_scientific
3
synthetic: yes
other_details: synthetic peptide
Authors : Li de la Sierra-Gallay, I., Fonvielle, M., van Tilbeurgh, H., Arthur, M., Etheve-Quelquejeu, M.
Keywords : FEMX, PEPTIDOGLYCAN, transferase, peptidyl-RNA conjugate complex, TRANSFERASE-PEPTIDE-RNA complex
Exp. method : X-RAY DIFFRACTION ( 1.66 Å )
Citation :

The Structure of FemXWv in Complex with a Peptidyl-RNA Conjugate: Mechanism of Aminoacyl Transfer from Ala-tRNA(Ala) to Peptidoglycan Precursors

Fonvielle, M.,Li de La Sierra-Gallay, I.,El-Sagheer, A.H.  et al.
(2013)  Angew.Chem.Int.Ed.Engl.  52 : 7278 - 7281

PubMed: 23744707
DOI: 10.1002/anie.201301411

Reaction

Chain : A
UniProt : Q9EY50 (FEMX_WEIVI)
Reaction: EC: Evidence:
Physiological Direction:
L-alanyl-tRNA(Ala) + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl- gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine = H(+) + tRNA(Ala) + UDP- N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-N(6)-(L-alanyl)- L-lysyl-D-alanyl-D-alanine 2.3.2.10 PubMed:11083873, PubMed:4248527
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