Brand  (β version)

PDB ID: 4IHG

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Ligands
Code Name Style Show Link
FTT 3-hydroxy-tetradecanoic acid PoSSuM
PNS 4'-phosphopantetheine PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 4IHG   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE/LIPID BINDING PROTEIN
Title : Chasing Acyl Carrier Protein Through a Catalytic Cycle of Lipid A Production
Release Data : 2013-11-13
Compound :
mol_id molecule chains synonym
1 UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase A,B,C,D,E,F UDP-3-O-(3-OHC14)-GlcN N-acyltransferase, Protein FirA, Rifampicin resistance protein
ec: 2.3.1.191
mol_id molecule chains synonym
2 Acyl carrier protein G,H,I,J,K,L ACP
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:83333) Escherichia coli  (taxid:562)
strain: K12
gene: lpxD, firA, omsA, b0179, JW0174
expression_system_strain: Rosetta (DE3)/pLysS
expression_system_vector_type: Plasmid
expression_system_plasmid: pET11d
mol_id organism_scientific expression_system
2 Escherichia coli  (taxid:885275) Escherichia coli  (taxid:562)
strain: 'clone D i14'
gene: acpP, i14_1248
expression_system_strain: C41(DE3)
expression_system_vector_type: Plasmid
expression_system_plasmid: pET16b
Authors : Masoudi, A., Raetz, C.R.H., Pemble, C.W.
Keywords : Left Handed Beta Helix, LpxD, acyltransferase, Lipid A, Protein-Protein complex, ACP Recognition Domain, ACP mediated product release, TRANSFERASE-LIPID BINDING PROTEIN complex
Exp. method : X-RAY DIFFRACTION ( 2.89 Å )
Citation :

Chasing acyl carrier protein through a catalytic cycle of lipid A production.

Masoudi, A.,Raetz, C.R.,Zhou, P.  et al.
(2014)  Nature  505 : 422 - 426

PubMed: 24196711
DOI: 10.1038/nature12679

Reaction

Chain : A, B, C, D, E, F
UniProt : P21645 (LPXD_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
a (3R)-hydroxyacyl-[ACP] + a UDP-3-O-[(3R)-3-hydroxyacyl]- alpha-D-glucosamine = a UDP-2-N,3-O-bis[(3R)-3-hydroxyacyl]-alpha-D- glucosamine + H(+) + holo-[ACP] 2.3.1.191 PubMed:18422345, PubMed:19655786, PubMed:8444173
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(3R)-hydroxytetradecanoyl-[ACP] + UDP-3-O-[(3R)-3- hydroxytetradecanoyl]-alpha-D-glucosamine = H(+) + holo-[ACP] + UDP- 2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-alpha-D-glucosamine - PubMed:18422345, PubMed:19655786, PubMed:8444173
-
Chain : G, H, I, J, K, L
UniProt : G7RM21
Reaction : -