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Ligands
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F4M 1-[4-({(1r)-1-[(6s,7r)-2-amino-5-formyl-7-methyl-4-oxo-1,4,5,6,7,8-hexahydropteridin-6-yl]ethyl}amino)phenyl]-1-deoxy-5 -O-{5-O-[(R)-{[(1r)-1,3-dicarboxypropyl]oxy}(hydroxy)phosphoryl]-alpha-D-ribofuranosyl}-D-ribitol
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Code : 4GVS   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : X-ray structure of the Archaeoglobus fulgidus methenyl-tetrahydromethanopterin cyclohydrolase in complex with N5-formyl-tetrahydromethanopterin
Release Data : 2012-10-31
Compound :
mol_id molecule chains synonym
1 Methenyltetrahydromethanopterin cyclohydrolase A,B,C Methenyl-H4MPT cyclohydrolase
ec: 3.5.4.27
mutation: E186Q
Source :
mol_id organism_scientific expression_system
1 Archaeoglobus fulgidus  (taxid:224325) Escherichia coli  (taxid:562)
strain: DSM 4304
gene: AF_1935, mch
expression_system_strain: Rosetta (DE3) pLysS
expression_system_vector_type: Plasmid
expression_system_plasmid: pET-24a(+)
Authors : Upadhyay, V., Demmer, U., Warkentin, E., Moll, J., Shima, S., Ermler, U.
Keywords : N5-formyl-tetrahydromethanopterin, HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 1.75 Å )
Citation :

Structure and catalytic mechanism of N(5),N(10)-methenyl-tetrahydromethanopterin cyclohydrolase.

Upadhyay, V.,Demmer, U.,Warkentin, E.  et al.
(2012)  Biochemistry  51 : 8435 - 8443

PubMed: 23013430
DOI: 10.1021/bi300777k

Chain : A, B, C
UniProt : O28344 (MCH_ARCFU)
Reaction: EC: Evidence:
Physiological Direction:
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O = H(+) + N(5)-formyl-5,6,7,8-tetrahydromethanopterin 3.5.4.27 -
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