Brand  (β version)

PDB ID: 4GRJ

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Ligands
Code Name Style Show Link
HEM Protoporphyrin ix containing Fe PoSSuM
NO Nitric oxide PoSSuM
PO4 Phosphate ion PoSSuM
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Glycosylation
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Structure summary

Code : 4GRJ   PDBj   RCSB PDB   PDBe
Header : TRANSPORT PROTEIN
Title : Crystal structure of nitrophorin 4 triple mutant complex with NO
Release Data : 2013-08-07
Compound :
mol_id molecule chains synonym
1 Nitrophorin-4 A,B NP4
mutation: D30N, E32Q, D35N
Source :
mol_id organism_scientific organism_common expression_system
1 Rhodnius prolixus  (taxid:13249) Triatomid bug Escherichia coli  (taxid:562)
expression_system_strain: BL21DE3
expression_system_vector_type: plasmid
expression_system_plasmid: pET17b
Authors : Weichsel, A., Issaian, A.V., Montfort, W.R.
Keywords : beta barrel, lipocalin, nitric oxide transport, nitric oxide binding, TRANSPORT PROTEIN
Exp. method : X-RAY DIFFRACTION ( 1.15 Å )
Citation :

Crystal structures of nitrophorin 4 triple mutant

Issaian, V.A.,Weichsel, A.,Montfort, W.R.
To be Published 

The crystal structure of nitrophorin 4 at 1.5 resolution: transport of nitric oxide by a lipocalin-based heme protein

Andersen, J.F.,Weichsel, A.,Balfour, C.A.  et al.
(1998)  Structure  6 : 1315 - 1327

Ultrahigh resolution structures of nitrophorin 4: Heme distortion in ferrous CO and NO complexes

Maes, E.M.,Roberts, S.A.,Weichsel, A.  et al.
(2005)  Biochemistry  44 : 12690 - 12699

Reaction

Chain : A, B
UniProt : Q94734 (NP4_RHOPR)
Reaction: EC: Evidence:
Physiological Direction:
2 H(+) + 3 nitrite = H2O + nitrate + 2 nitric oxide 1.7.6.1 PubMed:20524697
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