Brand  (β version)

PDB ID: 4G05

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Ligands
Code Name Style Show Link
CA Calcium ion PoSSuM
HEM Protoporphyrin ix containing Fe PoSSuM
JZ3 Guaiacol PoSSuM
SO4 Sulfate ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 4G05   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : The crystal structures of several mutants of Pleurotus eryngii versatile peroxidase
Release Data : 2012-10-24
Compound :
mol_id molecule chains synonym
1 Versatile peroxidase VPL2 A Versatile liquid phase peroxidase 2
ec: 1.11.1.16
fragment: RESIDUES 31-347
mutation: E140G, G191E
Source :
mol_id organism_scientific organism_common expression_system
1 Pleurotus eryngii  (taxid:5323) Boletus of the steppes Escherichia coli  (taxid:562)
gene: vpl2
Authors : Mate, M.J., Romero, A., Ruiz-Duenas, F.J., Martinez, A.T.
Keywords : LIGNIN PEROXIDASE, LIGNIN DEGRADATION, AROMATIC-SUBSTRATE BINDING, OXIDOREDUCTASE
Exp. method : X-RAY DIFFRACTION ( 2.35 Å )
Citation :

Two Oxidation Sites for Low Redox Potential Substrates: A DIRECTED MUTAGENESIS, KINETIC, AND CRYSTALLOGRAPHIC STUDY ON PLEUROTUS ERYNGII VERSATILE PEROXIDASE.

Morales, M.,Mate, M.J.,Romero, A.  et al.
(2012)  J.Biol.Chem.  287 : 41053 - 41067

PubMed: 23071108
DOI: 10.1074/jbc.M112.405548

Site-directed mutagenesis of the catalytic tryptophan environment in pleurotus eryngii versatile peroxidase

Ruiz-Duenas, F.J.,Morales, M.,Mate, M.J.  et al.
(2008)  Biochemistry  47 : 1685 - 1695

Reaction

Chain : A
UniProt : O94753 (VPL2_PLEER)
Reaction: EC: Evidence:
Physiological Direction:
1-(4-hydroxy-3-methoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3- diol + H2O2 = glycolaldehyde + guaiacol + H2O + vanillin 1.11.1.16 PubMed:12884090, PubMed:16246366, PubMed:9987124
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2 H(+) + H2O2 + 2 Mn(2+) = 2 H2O + 2 Mn(3+) 1.11.1.16 PubMed:12884090, PubMed:16246366, PubMed:9987124
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