Brand  (β version)

PDB ID: 4EKS

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Ligands
Code Name Style Show Link
BME Beta-mercaptoethanol PoSSuM
0R1 1,2-benzisoxazole PoSSuM
SO4 Sulfate ion PoSSuM
ACT Acetate ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 4EKS   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : T4 Lysozyme L99A/M102H with Isoxazole Bound
Release Data : 2012-09-05
Compound :
mol_id molecule chains synonym
1 Lysozyme A,B Endolysin, Lysis protein, Muramidase
ec: 3.2.1.17
mutation: T21C/S38D/L99A/M102H/E108V/S117V/T142C/N144D
Source :
mol_id organism_scientific expression_system
1 Enterobacteria phage T4  (taxid:10665) Escherichia coli  (taxid:469008)
gene: E
expression_system_strain: BL21(DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pET28
Authors : Merski, M., Shoichet, B.K.
Keywords : Hydrolase, Alkylation of Cys97
Exp. method : X-RAY DIFFRACTION ( 1.640 Å )
Citation :

Engineering a model protein cavity to catalyze the Kemp elimination.

Merski, M.,Shoichet, B.K.
(2012)  Proc.Natl.Acad.Sci.USA  109 : 16179 - 16183

PubMed: 22988064
DOI: 10.1073/pnas.1208076109

Reaction

Chain : A, B
UniProt : P00720 (ENLYS_BPT4)
Reaction: EC: Evidence:
Physiological Direction:
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins. 3.2.1.17 HAMAP-Rule:MF_04110, PubMed:4865643
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