Brand  (β version)

PDB ID: 4EBC

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Ligands
Code Name Style Show Link
0OH North-methanocarba-2'-deoxyadenosine triphosphate PoSSuM
CA Calcium ion PoSSuM
GOL Glycerol PoSSuM
Non-standard Residues
Code Name Show
DOC 2',3'-dideoxycytidine-5'-monophosphate
Glycosylation
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Modification
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Structure summary

Code : 4EBC   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE/DNA
Title : Conformationally Restrained North-methanocarba-2'-deoxyadenosine Corrects the Error-Prone Nature of Human DNA Polymerase Iota
Release Data : 2012-06-13
Compound :
mol_id molecule chains synonym
1 DNA polymerase iota A Eta2, RAD30 homolog B
ec: 2.7.7.7
fragment: UNP residues 26-445
mol_id molecule chains
2 5'-D(P*AP*GP*GP*AP*CP*CP*(DOC))-3' P
other_details: 7-mer primer DNA
mol_id molecule chains
3 5'-D(P*CP*TP*GP*GP*GP*TP*CP*CP*T)-3' T
other_details: 9-mer template DNA
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:469008)
gene: POLI, RAD30B
expression_system_strain: BL21(DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pBG101
mol_id organism_scientific
2
synthetic: yes
mol_id organism_scientific
3
synthetic: yes
Authors : Eoff, R.L., Ketkar, A., Banerjee, S.
Keywords : polymerase, TRANSFERASE-DNA complex
Exp. method : X-RAY DIFFRACTION ( 2.901 Å )
Citation :

A Nucleotide-Analogue-Induced Gain of Function Corrects the Error-Prone Nature of Human DNA Polymerase iota.

Ketkar, A.,Zafar, M.K.,Banerjee, S.  et al.
(2012)  J.Am.Chem.Soc.  134 : 10698 - 10705

PubMed: 22632140
DOI: 10.1021/ja304176q

Reaction

Chain : A
UniProt : Q9UNA4 (POLI_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate 2.7.7.7 PubMed:11013228, PubMed:11387224, PubMed:27555320
-
Cofactor: Evidence: Note:
Mn(2+) ECO:0000269 | PubMed:27555320
ECO:0000269 | PubMed:27555320
Binds nucleotide much more tightly and catalyzes nucleotide insertion much more efficiently in the presence of Mg(2+) than in the presence of Mn(2+).