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Ligands
Code Name Style Show Link
EDO 1,2-ethanediol
JHZ (2r,4s,6r)-4-amino-4,6-dimethyl-5-oxotetrahydro-2h-pyran-2-yl [(2r,3s,5r)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2h)-yl)tetrahydrofuran-2-yl]methyl dihydrogen diphosphate (non-preferred name)
SAH S-adenosyl-L-homocysteine
ZN Zinc ion
Non-standard Residues
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Glycosylation
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Code : 4E2W   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : X-ray Structure of the H181N mutant of TcaB9, a C-3'-Methyltransferase, in Complex with S-Adenosyl-L-Homocysteine and Sugar Product
Release Data : 2012-04-25
Compound :
mol_id molecule chains synonym
1 TcaB9 A C-3'-Methyltransferase
ec: 2.1.1.-
mutation: H181N
Source :
mol_id organism_scientific expression_system
1 Micromonospora chalcea  (taxid:1874) Escherichia coli  (taxid:562)
gene: tcab9
expression_system_strain: Rosetta2 DE3
expression_system_vector_type: plasmid
expression_system_plasmid: pET28
Authors : Bruender, N.A., Holden, H.M.
Keywords : kijanose, tetronitrose, tetradeoxy sugar, keto sugar, sugar methylation, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 1.50 Å )
Citation :

Probing the catalytic mechanism of a C-3'-methyltransferase involved in the biosynthesis of D-tetronitrose.

Bruender, N.A.,Holden, H.M.
(2012)  Protein Sci.  21 : 876 - 886

PubMed: 22495991
DOI: 10.1002/pro.2074

Chain : A
UniProt : B5L6K6 (B5L6K6_MICCH)
Reaction : -