Brand  (β version)

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain:

Ligands
Code Name Link
0L9 (2s)-[6-bromo-4-(4-chlorophenyl)-2-methylquinolin-3-yl](methoxy)ethanoic acid
ARS Arsenic
SO4 Sulfate ion
Code : 4DMN
Header : VIRAL PROTEIN/Inhibitor
Title : HIV-1 Integrase Catalytical Core Domain
Release Data : 2012-03-21
Compound :
mol_id molecule chains synonym
1 HIV-1 Integrase A IN
Source :
mol_id organism_scientific organism_common expression_system
1 Human immunodeficiency virus type 1  (taxid:11698) HIV-1 Escherichia coli  (taxid:469008)
gene: gag-pol
expression_system_strain: BL21(DE3)
expression_system_vector_type: plasmid
Authors : Feng, L., Kvaratskhelia, M.
Keywords : Integrase, CCD, DDE motif, dimer interface, VIRAL PROTEIN-Inhibitor complex
Exp. method : X-RAY DIFFRACTION ( 2.4500 Å )
Citation :

Multimode, cooperative mechanism of action of allosteric HIV-1 integrase inhibitors.

Kessl, J.J.,Jena, N.,Koh, Y.  et al.
(2012)  J.Biol.Chem.  287 : 16801 - 16811

PubMed: 22437836
DOI: 10.1074/jbc.M112.354373

Chain : A
UniProt : P12497 (POL_HV1N5)
Reaction : Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid.
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).