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Ligands
Code Name Style Show Link
0GY (1e,3z)-4-hydroxybuta-1,3-diene-1,2,4-tricarboxylic acid
ACT Acetate ion
Non-standard Residues
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Glycosylation
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Code : 4DI9   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : CRYSTAL STRUCTURE OF THE D248A mutant of 2-PYRONE-4,6-DICARBOXYLIC ACID HYDROLASE FROM SPHINGOMONAS PAUCIMOBILIS complexed with substrate at pH 6.5
Release Data : 2012-10-03
Compound :
mol_id molecule chains synonym
1 2-pyrone-4,6-dicarbaxylate hydrolase A 2-pyrone-4,6-dicarboxylic acid hydrolase
mutation: D246A
Source :
mol_id organism_scientific expression_system
1 Sphingomonas paucimobilis  (taxid:13689) Escherichia coli  (taxid:469008)
strain: SYK-6
gene: ligI
expression_system_strain: BL21(DE3)CODON+RIL
expression_system_vector_type: plasmid
expression_system_plasmid: BC-PSGX3(BC)
Authors : Malashkevich, V.N., Toro, R., Hobbs, M.E., Raushel, F.M., Almo, S.C.
Keywords : HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 1.3500 Å )
Citation :

Structure and Catalytic Mechanism of LigI: Insight into the Amidohydrolase Enzymes of cog3618 and Lignin Degradation.

Hobbs, M.E.,Malashkevich, V.,Williams, H.J.  et al.
(2012)  Biochemistry  51 : 3497 - 3507

PubMed: 22475079
DOI: 10.1021/bi300307b

Chain : A
UniProt : O87170 (LIGI_SPHSK)
Reaction: EC: Evidence:
Physiological Direction:
2-oxo-2H-pyran-4,6-dicarboxylate + H2O = (1E)-4-oxobut-1-ene- 1,2,4-tricarboxylate + H(+) 3.1.1.57 PubMed:22475079, PubMed:29658701
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