Brand  (β version)

PDB ID: 4DF4

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Ligands
Code Name Style Show Link
0L3 7-{2-deoxy-5-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]oxy}phosphoryl]-beta-D-erythro-pentofuranosyl}-5-{5-[(10-hydroxydecanoyl)amino]pent-1-yn-1-yl}-7h-pyrrolo[2,3-D]pyrimidin-4-amine PoSSuM
EDO 1,2-ethanediol PoSSuM
MG Magnesium ion PoSSuM
Non-standard Residues
Code Name Show
DOC 2',3'-dideoxycytidine-5'-monophosphate
Glycosylation
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Modification
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Structure summary

Code : 4DF4   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE/DNA
Title : Crystal structure of the large fragment of DNA Polymerase I from Thermus aquaticus in a closed ternary complex with 7-(N-(10-hydroxydecanoyl)-aminopentinyl)-7-deaza-2 -dATP
Release Data : 2012-05-16
Compound :
mol_id molecule chains synonym
1 DNA polymerase I, thermostable A Taq polymerase 1
ec: 2.7.7.7
fragment: Klenow Fragment
mol_id molecule chains
2 DNA (5'-D(*GP*AP*CP*CP*AP*CP*GP*GP*CP*GP*CP*(DOC))-3') B
fragment: DNA Primer
mol_id molecule chains
3 DNA (5'-D(*AP*AP*AP*TP*GP*GP*CP*GP*CP*CP*GP*TP*GP*GP*TP*C)-3') C
fragment: DNA Template
Source :
mol_id organism_scientific expression_system
1 Thermus aquaticus  (taxid:271) Escherichia coli  (taxid:562)
gene: pol I, pol1, polA
expression_system_strain: Bl21-DE3
expression_system_vector_type: pET series
expression_system_plasmid: pEt21b
mol_id organism_scientific
2
synthetic: yes
mol_id organism_scientific
3
synthetic: yes
Authors : Bergen, K., Steck, A., Struett, S., Baccaro, A., Welte, W., Diederichs, K., Marx, A.
Keywords : DNA Polymerase, TRANSFERASE-DNA complex
Exp. method : X-RAY DIFFRACTION ( 2.200 Å )
Citation :

Structures of KlenTaq DNA Polymerase Caught While Incorporating C5-Modified Pyrimidine and C7-Modified 7-Deazapurine Nucleoside Triphosphates.

Bergen, K.,Steck, A.L.,Strutt, S.  et al.
(2012)  J.Am.Chem.Soc.  134 : 11840 - 11843

PubMed: 22475415
DOI: 10.1021/ja3017889

Reaction

Chain : A
UniProt : P19821 (DPO1_THEAQ)
Reaction: EC: Evidence:
Physiological Direction:
a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1) 2.7.7.7 -
-