Brand  (β version)

PDB ID: 4DA5

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Ligands
Code Name Style Show Link
0H7 (3r)-1-azabicyclo[2.2.2]oct-3-yl[bis(5-chlorothiophen-2-yl)]methanol PoSSuM
SO4 Sulfate ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 4DA5   PDBj   RCSB PDB   PDBe
Header : Transferase/Transferase Inhibitor
Title : Choline Kinase alpha acts through a double-displacement kinetic mechanism involving enzyme isomerisation, as determined through enzyme and inhibitor kinetics and structural biology
Release Data : 2013-04-17
Compound :
mol_id molecule chains synonym
1 Choline kinase alpha A,B CK, CHETK-alpha, Ethanolamine kinase;, EK
ec: 2.7.1.32, 2.7.1.82
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:469008)
gene: CHKA, CHK, CKI
expression_system_strain: BL21(DE3)
expression_system_vector_type: modified pET28a
Authors : Brown, K., Hudson, C., Charlton, P., Pollard, J.
Keywords : kinase, signal transduction, cytoplasmic, Transferase-Transferase Inhibitor complex
Exp. method : X-RAY DIFFRACTION ( 2.40 Å )
Citation :

Kinetic and mechanistic characterisation of Choline Kinase-alpha.

Hudson, C.S.,Knegtel, R.M.,Brown, K.  et al.
(2013)  Biochim.Biophys.Acta  1834 : 1107 - 1116

PubMed: 23416529
DOI: 10.1016/j.bbapap.2013.02.008

Reaction

Chain : A, B
UniProt : P35790 (CHKA_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
ATP + choline = ADP + H(+) + phosphocholine 2.7.1.32 PubMed:17007874, PubMed:19915674, PubMed:34077757, PubMed:35202461
left-to-right PubMed:17007874, PubMed:19915674
ATP + ethanolamine = ADP + H(+) + phosphoethanolamine 2.7.1.82 PubMed:17007874, PubMed:19915674
left-to-right PubMed:17007874, PubMed:19915674
[Isoform 1]
ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl- [protein] 		- PubMed:34077757
left-to-right