Brand  (β version)

PDB ID: 4ZYR

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Ligands
Code Name Style Show Link
9PG 4-nitrophenyl alpha-D-galactopyranoside PoSSuM
BNG Nonyl beta-D-glucopyranoside PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 4ZYR   PDBj   RCSB PDB   PDBe
Header : TRANSPORT PROTEIN
Title : Crystal structure of E. coli Lactose permease G46W/G262W bound to P-nitrophenyl alpha-D-galactopyranoside (alpha-NPG)
Release Data : 2015-07-29
Compound :
mol_id molecule chains synonym
1 Lactose permease A,B Lactose-proton symport
mutation: G46W, G262W
Source :
mol_id organism_scientific expression_system
1 Escherichia coli (strain K12)  (taxid:83333) ESCHERICHIA COLI  (taxid:562)
strain: K12
gene: lacY, b0343, JW0334
expression_system_strain: C41
expression_system_vector_type: Plasmid
expression_system_plasmid: pT7-5
Authors : Kumar, H., Finer-Moore, J.S., Kaback, H.R., Stroud, R.M.
Keywords : Membrane Protein, Transporter, Alpha-Helical, Major Facilitator Superfamily (MFS), TRANSPORT PROTEIN
Exp. method : X-RAY DIFFRACTION ( 3.3120 Å )
Citation :

Structure of LacY with an alpha-substituted galactoside: Connecting the binding site to the protonation site.

Kumar, H.,Finer-Moore, J.S.,Kaback, H.R.  et al.
(2015)  Proc.Natl.Acad.Sci.USA  112 : 9004 - 9009

PubMed: 26157133
DOI: 10.1073/pnas.1509854112

Reaction

Chain : A, B
UniProt : P02920 (LACY_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
H(+)(in) + lactose(in) = H(+)(out) + lactose(out) - PubMed:1848449, PubMed:7000781, PubMed:7028742
right-to-left
H(+)(in) + melibiose(in) = H(+)(out) + melibiose(out) - PubMed:1848449
right-to-left