Brand  (β version)

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain:

Ligands
Code Name Link
CLX Tri-chloro-acetaldehyde
SO4 Sulfate ion
Code : 4YAS
Header : LYASE
Title : HYDROXYNITRILE LYASE COMPLEXED WITH CHLORALHYDRATE
Release Data : 1999-10-13
Compound :
mol_id molecule chains synonym
1 PROTEIN (HYDROXYNITRILE LYASE) A OXYNITRILASE
ec: 4.1.2.39
Source :
mol_id organism_scientific expression_system
1 Hevea brasiliensis  (taxid:3981) Pichia pastoris  (taxid:4922)
organ: LEAF
gene: HNL
expression_system_plasmid: BHIL-D2
expression_system_gene: HNL
Authors : Zuegg, J., Wagner, U.G., Gugganig, M., Kratky, C.
Keywords : OXYNITRILASE, CYANOGENESIS, CYANOHYDRIN FORMATION, LYASE
Exp. method : X-RAY DIFFRACTION ( 2.00 Å )
Citation :

Three-dimensional structures of enzyme-substrate complexes of the hydroxynitrile lyase from Hevea brasiliensis.

Zuegg, J.,Gruber, K.,Gugganig, M.  et al.
(1999)  Protein Sci.  8 : 1990 - 2000

PubMed: 10548044
DOI: 10.1110/ps.8.10.1990

Hydroxynitrile lyase from Hevea brasiliensis: molecular characterization and mechanism of enzyme catalysis.

Hasslacher, M.,Kratky, C.,Griengl, H.  et al.
(1997)  Proteins  27 : 438 - 449

PubMed: 9094745
DOI: 10.1002/(SICI)1097-0134(199703)27:3<438::AID-PROT11>3.0.CO;2-M

Mechanism of cyanogenesis: the crystal structure of hydroxynitrile lyase from Hevea brasiliensis.

Wagner, U.G.,Hasslacher, M.,Griengl, H.  et al.
(1996)  Structure  4 : 811 - 822

PubMed: 8805565
DOI: 10.1016/s0969-2126(96)00088-3

Crystallization and preliminary X-ray diffraction studies of a hydroxynitrile lyase from Hevea brasiliensis.

Wagner, U.G.,Schall, M.,Hasslacher, M.  et al.
(1996)  Acta Crystallogr.,Sect.D  52 : 591 - 593

PubMed: 15299689
DOI: 10.1107/S0907444995016830

Molecular Cloning of the Full-Length Cdna of (S)-Hydroxynitrile Lyase from Hevea brasiliensis. Functional Expression in Escherichia coli and Saccharomyces cerevisiae and Identification of an Active Site Residue

Hasslacher, M.,Schall, M.,Hayn, M.  et al.
(1996)  J.Biol.Chem.  271 : 5884 - 5891

PubMed: 8621461
DOI: 10.1074/jbc.271.10.5884

Chain : A
UniProt : P52704 (HNL_HEVBR)
Reaction : An aliphatic (S)-hydroxynitrile = cyanide + an aliphatic aldehyde or ketone.
An aromatic (S)-hydroxynitrile = cyanide + an aromatic aldehyde.