Brand  (β version)

PDB ID: 4YAS

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Ligands
Code Name Style Show Link
SO4 Sulfate ion PoSSuM
CLX Tri-chloro-acetaldehyde PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 4YAS   PDBj   RCSB PDB   PDBe
Header : LYASE
Title : HYDROXYNITRILE LYASE COMPLEXED WITH CHLORALHYDRATE
Release Data : 1999-10-13
Compound :
mol_id molecule chains synonym
1 PROTEIN (HYDROXYNITRILE LYASE) A OXYNITRILASE
ec: 4.1.2.39
Source :
mol_id organism_scientific expression_system
1 Hevea brasiliensis  (taxid:3981) Pichia pastoris  (taxid:4922)
organ: LEAF
gene: HNL
expression_system_plasmid: BHIL-D2
expression_system_gene: HNL
Authors : Zuegg, J., Wagner, U.G., Gugganig, M., Kratky, C.
Keywords : OXYNITRILASE, CYANOGENESIS, CYANOHYDRIN FORMATION, LYASE
Exp. method : X-RAY DIFFRACTION ( 2.00 Å )
Citation :

Three-dimensional structures of enzyme-substrate complexes of the hydroxynitrile lyase from Hevea brasiliensis.

Zuegg, J.,Gruber, K.,Gugganig, M.  et al.
(1999)  Protein Sci.  8 : 1990 - 2000

PubMed: 10548044
DOI: 10.1110/ps.8.10.1990

Hydroxynitrile lyase from Hevea brasiliensis: molecular characterization and mechanism of enzyme catalysis.

Hasslacher, M.,Kratky, C.,Griengl, H.  et al.
(1997)  Proteins  27 : 438 - 449

PubMed: 9094745
DOI: 10.1002/(SICI)1097-0134(199703)27:3<438::AID-PROT11>3.0.CO;2-M

Mechanism of cyanogenesis: the crystal structure of hydroxynitrile lyase from Hevea brasiliensis.

Wagner, U.G.,Hasslacher, M.,Griengl, H.  et al.
(1996)  Structure  4 : 811 - 822

PubMed: 8805565
DOI: 10.1016/s0969-2126(96)00088-3

Crystallization and preliminary X-ray diffraction studies of a hydroxynitrile lyase from Hevea brasiliensis.

Wagner, U.G.,Schall, M.,Hasslacher, M.  et al.
(1996)  Acta Crystallogr.,Sect.D  52 : 591 - 593

PubMed: 15299689
DOI: 10.1107/S0907444995016830

Molecular Cloning of the Full-Length Cdna of (S)-Hydroxynitrile Lyase from Hevea brasiliensis. Functional Expression in Escherichia coli and Saccharomyces cerevisiae and Identification of an Active Site Residue

Hasslacher, M.,Schall, M.,Hayn, M.  et al.
(1996)  J.Biol.Chem.  271 : 5884 - 5891

PubMed: 8621461
DOI: 10.1074/jbc.271.10.5884

Reaction

Chain : A
UniProt : P52704 (HNL_HEVBR)
Reaction: EC: Evidence:
Physiological Direction:
a monosubstituted aliphatic (S)-hydroxynitrile = an aldehyde + hydrogen cyanide 4.1.2.47 Ref.2, Ref.4
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a disubstituted aliphatic (S)-hydroxynitrile = a ketone + hydrogen cyanide 4.1.2.47 ECO:0000305
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an aromatic (S)-hydroxynitrile = an aromatic aldehyde + hydrogen cyanide 4.1.2.47 Ref.2, Ref.4
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2-hydroxy-2-methylpropanenitrile = acetone + hydrogen cyanide - Ref.2, Ref.3
left-to-right Ref.3
butan-2-one + hydrogen cyanide = 2-hydroxy-2- methylbutanenitrile - Ref.3
right-to-left
benzaldehyde + hydrogen cyanide = (S)-mandelonitrile - Ref.2, Ref.4, PubMed:14998991, PubMed:8621461, PubMed:9094745
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butanal + hydrogen cyanide = (2S)-2-hydroxypentanenitrile - Ref.2
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2-methylpropanal + hydrogen cyanide = (2S)-2-hydroxy-3- methylbutanenitrile - Ref.2
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2,2-dimethylpropanal + hydrogen cyanide = (2S)-2-hydroxy-3,3- dimethylbutanenitrile - Ref.2
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hexanal + hydrogen cyanide = (2S)-2-hydroxyheptanenitrile - Ref.2
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nonanal + hydrogen cyanide = (2S)-2-hydroxydecanenitrile - Ref.2
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acrolein + hydrogen cyanide = (2S)-2-hydroxybut-3-enenitrile - Ref.2
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2-phenylacetaldehyde + hydrogen cyanide = (2S)-2-hydroxy-3- phenylpropanenitrile - Ref.4
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3-phenylpropanal + hydrogen cyanide = (2S)-2-hydroxy-4- phenylbutanenitrile - Ref.4
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(E)-cinnamaldehyde + hydrogen cyanide = (2S,3E)-2-hydroxy-4- phenylbut-3-enenitrile - Ref.4
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2-methoxybenzaldehyde + hydrogen cyanide = (2S)-2-hydroxy-2- (2-methoxyphenyl)acetonitrile - Ref.4
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3-methoxybenzaldehyde + hydrogen cyanide = (2S)-2-hydroxy-2- (3-methoxyphenyl)acetonitrile - Ref.4
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4-methoxybenzaldehyde + hydrogen cyanide = (2S)-2-hydroxy-2- (4-methoxyphenyl)acetonitrile - Ref.4
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cyclohexanecarbaldehyde + hydrogen cyanide = (2S)-2- cyclohexyl-2-hydroxyacetonitrile - Ref.4
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furfural + hydrogen cyanide = (2R)-2-(furan-2-yl)-2- hydroxyacetonitrile - Ref.4
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formylthiophene + hydrogen cyanide = (2R)-2-hydroxy-2- (thiophen-2-yl)acetonitrile - Ref.4
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3-formylthiophene + hydrogen cyanide = (2S)-2-hydroxy-2- (thiophen-3-yl)acetonitrile - Ref.4
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