Brand  (β version)

PDB ID: 4WX2

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Ligands
Code Name Style Show Link
EDO 1,2-ethanediol PoSSuM
F6F 2-{[4-(trifluoromethoxy)benzoyl]amino}ethyl dihydrogen phosphate PoSSuM
NA Sodium ion PoSSuM
PEG Di(hydroxyethyl)ether PoSSuM
PLP Pyridoxal-5'-phosphate PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 4WX2   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Crystal structure of Tryptophan Synthase from Salmonella typhimurium in complex with two F6F molecules in the alpha-site and one F6F molecule in the beta-site
Release Data : 2015-11-18
Compound :
mol_id molecule chains
1 Tryptophan synthase alpha chain A
ec: 4.2.1.20
mol_id molecule chains
2 Tryptophan synthase beta chain B
ec: 4.2.1.20
Source :
mol_id organism_scientific expression_system
1 Salmonella enterica subsp. enterica serovar Typhimurium  (taxid:90371) Escherichia coli  (taxid:562)
gene: trpA
expression_system_strain: Cb149
expression_system_vector_type: plasmid
expression_system_plasmid: Derivative of Pbr322
mol_id organism_scientific expression_system
2 Salmonella enterica subsp. enterica serovar Typhimurium  (taxid:90371) Escherichia coli  (taxid:562)
gene: trpB
expression_system_strain: Cb149
expression_system_vector_type: plasmid
expression_system_plasmid: Derivative of Pbr322
Authors : Hilario, E., Caulkins, B.G., Young, R.P., Niks, D., Dunn, M.F., Mueller, L.J., Fan, L.
Keywords : carbon-oxygen lyase, tryptophan biosynthesis, Salmonella typhimurium, F6F, inhibitor, allosteric enzyme, aromatic amino acid biosynthesis, pyridoxal phosphate, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 1.7500 Å )
Citation :

Visualizing the tunnel in tryptophan synthase with crystallography: Insights into a selective filter for accommodating indole and rejecting water.

Hilario, E.,Caulkins, B.G.,Huang, Y.M.  et al.
(2016)  Biochim.Biophys.Acta  1864 : 268 - 279

PubMed: 26708480
DOI: 10.1016/j.bbapap.2015.12.006

Reaction

Chain : A
UniProt : P00929 (TRPA_SALTY)
Reaction: EC: Evidence:
Physiological Direction:
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D- glyceraldehyde 3-phosphate + H2O + L-tryptophan 4.2.1.20 HAMAP-Rule:MF_00131
-
Chain : B
UniProt : P0A2K1 (TRPB_SALTY)
Reaction: EC: Evidence:
Physiological Direction:
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D- glyceraldehyde 3-phosphate + H2O + L-tryptophan 4.2.1.20 -
-