Brand  (β version)

PDB ID: 4TRI

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Ligands
Code Name Style Show Link
HEM Protoporphyrin ix containing Fe PoSSuM
C3S Cholest-5-en-3-yl hydrogen sulfate PoSSuM
GOL Glycerol PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 4TRI   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : X-ray crystal structure of CYP142A2 from Mycobacterium smegmatis, complexed with cholesterol sulfate.
Release Data : 2014-09-17
Compound :
mol_id molecule chains
1 P450 heme-thiolate protein A,B
Source :
mol_id organism_scientific expression_system
1 Mycobacterium smegmatis  (taxid:246196) Escherichia coli  (taxid:562)
strain: ATCC 700084 / mc(2)155
gene: MSMEG_5918
expression_system_plasmid: pCWori
Authors : Madrona, Y., Ortiz de Montellano, P.
Keywords : Cytochrome P450, cholesterol sulfate, ligand bound, OXIDOREDUCTASE
Exp. method : X-RAY DIFFRACTION ( 2.00 Å )
Citation :

Cholesterol ester oxidation by mycobacterial cytochrome p450.

Frank, D.J.,Madrona, Y.,Ortiz de Montellano, P.R.
(2014)  J.Biol.Chem.  289 : 30417 - 30425

PubMed: 25210044
DOI: 10.1074/jbc.M114.602771

Reaction

Chain : A, B
UniProt : A0R4Q6 (CP142_MYCS2)
Reaction: EC: Evidence:
Physiological Direction:
cholest-4-en-3-one + 6 reduced [2Fe-2S]-[ferredoxin] + 3 O2 + 5 H(+) = (25R)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S]- [ferredoxin] + 4 H2O 1.14.15.28 PubMed:23489718, PubMed:25210044
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Cofactor: Evidence: Note:
heme ECO:0000269 | PubMed:23489718
ECO:0000269 | PubMed:25210044
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