Brand  (β version)

PDB ID: 4S23

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Ligands
Code Name Style Show Link
H6P (2e)-4-hydroxy-3-methylbut-2-en-1-yl trihydrogen diphosphate PoSSuM
MES 2-(N-morpholino)-ethanesulfonic acid PoSSuM
SF4 Iron/sulfur cluster PoSSuM
Non-standard Residues
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Structure summary

Code : 4S23   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Structure of the GcpE-HMBPP complex from Thermus thermophilius
Release Data : 2015-03-25
Compound :
mol_id molecule chains synonym
1 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase A,B 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase
ec: 1.17.7.1
Source :
mol_id organism_scientific expression_system
1 Thermus thermophilus  (taxid:274) Escherichia coli  (taxid:562)
gene: ispG, gcpE
expression_system_vector_type: plasmid
expression_system_plasmid: pQE-60
Authors : Rekittke, I., Warkentin, E., Jomaa, H., Ermler, E.
Keywords : TIM barrel, alpha/beta fold, OXIDOREDUCTASE
Exp. method : X-RAY DIFFRACTION ( 1.650 Å )
Citation :

Structure of the GcpE-HMBPP complex from Thermus thermophilius.

Rekittke, I.,Warkentin, E.,Jomaa, H.  et al.
(2015)  Biochem.Biophys.Res.Commun.  458 : 246 - 250

PubMed: 25660452
DOI: 10.1016/j.bbrc.2015.01.088

Reaction

Chain : A, B
UniProt : Q84GJ3 (ISPG_THETH)
Reaction: EC: Evidence:
Physiological Direction:
(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + oxidized [flavodoxin] + H2O + 2 H(+) = 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + reduced [flavodoxin] 1.17.7.3 HAMAP-Rule:MF_00159, PubMed:12482607
-
Cofactor: Evidence: Note:
[4Fe-4S] cluster ECO:0000255 | HAMAP-Rule:MF_00159
ECO:0000269 | PubMed:12482607
Binds 1 [4Fe-4S] cluster.