Brand  (β version)

PDB ID: 4PUJ

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Ligands
Code Name Style Show Link
CKR 6-amino-2-[(2-morpholin-4-ylethyl)amino]-3,7-dihydro-8h-imidazo[4,5-G]quinazolin-8-one PoSSuM
CL Chloride ion PoSSuM
GOL Glycerol PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
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Structure summary

Code : 4PUJ   PDBj   RCSB PDB   PDBe
Header : transferase/transferase inhibitor
Title : tRNA-Guanine Transglycosylase (TGT) in Complex with 6-Amino-2-{[2-(morpholin-4-yl)ethyl]amino}-1H,7H,8H-imidazo[4,5-G]quinazolin-8-one
Release Data : 2014-07-16
Compound :
mol_id molecule chains synonym
1 Queuine tRNA-ribosyltransferase A Guanine insertion enzyme, tRNA-guanine transglycosylase
ec: 2.4.2.29
fragment: Guanine Insertion Enzyme
Source :
mol_id organism_scientific expression_system
1 Zymomonas mobilis subsp. mobilis  (taxid:264203) Escherichia coli  (taxid:469008)
strain: ATCC 31821 / ZM4 / CP4
gene: tgt, ZMO0363
expression_system_strain: BL21(DE3)pLysS
expression_system_vector_type: Plasmid
expression_system_plasmid: pET9d-ZM4
Authors : Neeb, M., Heine, A., Klebe, G.
Keywords : Transferase, Guanine Exchange Enzyme, Guanine, preQ1, tRNA, transferase-transferase inhibitor complex
Exp. method : X-RAY DIFFRACTION ( 1.42 Å )
Citation :

Chasing Protons: How Isothermal Titration Calorimetry, Mutagenesis, and pKa Calculations Trace the Locus of Charge in Ligand Binding to a tRNA-Binding Enzyme.

Neeb, M.,Czodrowski, P.,Heine, A.  et al.
(2014)  J.Med.Chem.  57 : 5554 - 5565

PubMed: 24955548
DOI: 10.1021/jm500401x

Reaction

Chain : A
UniProt : P28720 (TGT_ZYMMO)
Reaction: EC: Evidence:
Physiological Direction:
7-aminomethyl-7-carbaguanine + guanosine(34) in tRNA = 7- aminomethyl-7-carbaguanosine(34) in tRNA + guanine 2.4.2.29 HAMAP- Rule:MF_00168, PubMed:7665516
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