Brand  (β version)

PDB ID: 4PFH

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Ligands
Code Name Style Show Link
MN Manganese (II) ion PoSSuM
FUD D-fructose PoSSuM
PSJ D-psicose PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 4PFH   PDBj   RCSB PDB   PDBe
Header : ISOMERASE
Title : Crystal structure of engineered D-tagatose 3-epimerase PcDTE-IDF8
Release Data : 2014-10-22
Compound :
mol_id molecule chains
1 D-tagatose 3-epimerase A,B
ec: 5.3.1.-
fragment: Isomerase
mutation: S37N,G39E,T109N,S116H,K122V,F157Y,T194N,H209V,L212I,K251T,S256G,A258D,G260C,M265L
Source :
mol_id organism_scientific expression_system
1 Pseudomonas cichorii  (taxid:36746) Escherichia coli  (taxid:469008)
expression_system_strain: BL21(DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pKTS-C6H
Authors : Hee, C.S., Bosshart, A., Schirmer, T.
Keywords : epimerase, TIM-barrel, ISOMERASE
Exp. method : X-RAY DIFFRACTION ( 1.90 Å )
Citation :

Directed Divergent Evolution of a Thermostable D-Tagatose Epimerase towards Improved Activity for Two Hexose Substrates.

Bosshart, A.,Hee, C.S.,Bechtold, M.  et al.
(2015)  Chembiochem  16 : 592 - 601

PubMed: 25655925
DOI: 10.1002/cbic.201402620

Reaction

Chain : A, B
UniProt : O50580 (DT3E_PSECI)
Reaction: EC: Evidence:
Physiological Direction:
keto-D-tagatose = keto-D-sorbose 5.1.3.31 PubMed:25655925, Ref.2
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D-allulose = keto-D-fructose 5.1.3.31 PubMed:25655925, Ref.2
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D-ribulose = D-xylulose 5.1.3.31 Ref.2
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Cofactor: Evidence: Note:
Mn(2+) ECO:0000269 | PubMed:17936787
ECO:0000269 | PubMed:25655925
ECO:0000269 | Ref.2
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