Brand  (β version)

PDB ID: 4P4F

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Ligands
Code Name Style Show Link
2GB N-[(S)-(biphenyl-4-ylmethoxy)(hydroxy)phosphoryl]-L-glutamic acid PoSSuM
CA Calcium ion PoSSuM
CL Chloride ion PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
Code Name Show
Glycosylation
Code Name Emphasize
BMA Beta-D-mannopyranose
MAN Alpha-D-mannopyranose
NAG 2-acetamido-2-deoxy-beta-D-glucopyranose
Modification
Code Name Show

Structure summary

Code : 4P4F   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : X-ray structure of human glutamate carboxypeptidase II (GCPII) in complex with a phosphoramidate inhibitor NC-2-40
Release Data : 2015-05-20
Compound :
mol_id molecule chains synonym
1 Glutamate carboxypeptidase 2 A Cell growth-inhibiting gene 27 protein,Folate hydrolase 1,Folylpoly-gamma-glutamate carboxypeptidase,FGCP,Glutamate carboxypeptidase II,GCPII,Membrane glutamate carboxypeptidase,mGCP,N-acetylated-alpha-linked acidic dipeptidase I,NAALADase I,Prostate-specific membrane antigen,PSMA,Pteroylpoly-gamma-glutamate carboxypeptidase
ec: 3.4.17.21
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Drosophila melanogaster  (taxid:7227)
gene: FOLH1, FOLH, NAALAD1, PSM, PSMA, GIG27
expression_system_common: Fruit fly
expression_system_cell_line: Schneider's S2 cells
expression_system_vector_type: plasmid
Authors : Barinka, C.
Keywords : glutamate carboxypeptidase II (GCPII); NAALADase; prostate-specific membrane antigen; phosphoramidate, hydrolase
Exp. method : X-RAY DIFFRACTION ( 1.86 Å )
Citation :

Design of composite inhibitors targeting glutamate carboxypeptidase II: the importance of effector functionalities.

Novakova, Z.,Cerny, J.,Choy, C.J.  et al.
(2016)  Febs J.  283 : 130 - 143

PubMed: 26460595
DOI: 10.1111/febs.13557

Reaction

Chain : A
UniProt : Q04609 (FOLH1_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
Release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates. 3.4.17.21 -
-
Cofactor: Evidence: Note:
Zn(2+) ECO:0000269 | PubMed:16467855
ECO:0000269 | PubMed:17372356
ECO:0000269 | PubMed:17567119
ECO:0000269 | PubMed:18234225
ECO:0000269 | PubMed:19053759
ECO:0000269 | PubMed:19301871
Binds 2 Zn(2+) ions per subunit. Required for NAALADase activity.