Brand  (β version)

PDB ID: 4O9R

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Ligands
Code Name Style Show Link
CY8 Cyclopamine PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 4O9R   PDBj   RCSB PDB   PDBe
Header : SIGNALING PROTEIN
Title : Human Smoothened Receptor structure in complex with cyclopamine
Release Data : 2014-03-05
Compound :
mol_id molecule chains
1 Smoothened homolog/Soluble cytochrome b562 chimeric protein A
fragment: UNP Q99835 residues 190-433, P0ABE7 residues 23-128, Q99835 residues 441-555
mutation: M1007W, H1102I, R1106L
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:562) Spodoptera frugiperda  (taxid:7108)
expression_system_strain: sf9
expression_system_plasmid: pFASTBAC
Authors : Wang, C., Weierstall, U., James, D., White, T.A., Wang, D., Liu, W., Spence, J.C.H., Doak, R.B., Nelson, G., Fromme, P., Fromme, R., Grotjohann, I., Kupitz, C., Zatsepin, N.A., Liu, H., Basu, S., Wacker, D., Han, G.W., Katritch, V., Boutet, S., Messerschmidt, M., Willams, G.J., Koglin, J.E., Seibert, M.M., Klinker, M., Gati, C., Shoeman, R.L., Barty, A., Chapman, H.N., Kirian, R.A., Beyerlein, K.R., Stevens, R.C., Li, D., Shah, S.T.A., Howe, N., Caffrey, M., Cherezov, V., GPCR Network (GPCR)
Keywords : serial femtosecond crystallography, human SMO receptor, cyclopamine, novel protein engineering, GPCR network, PSI-Biology, free electron laser, Structural Genomics, membrane protein, XFEL, LCP, room temperature structure, GPCR, membrane, SIGNALING PROTEIN
Exp. method : X-RAY DIFFRACTION ( 3.204 Å )
Citation :

Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography.

Weierstall, U.,James, D.,Wang, C.  et al.
(2014)  Nat Commun  5 : 3309 - 3309

PubMed: 24525480
DOI: 10.1038/ncomms4309

Reaction

Chain : A
UniProt : Q99835 (SMO_HUMAN)
Reaction : -
Chain : A
UniProt : P0ABE7 (C562_ECOLX)
Reaction : -