Brand  (β version)

PDB ID: 4NLK

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Ligands
Code Name Style Show Link
0KX 2'-deoxy-5'-O-[(R)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]cytidine PoSSuM
MG Magnesium ion PoSSuM
NA Sodium ion PoSSuM
Non-standard Residues
Code Name Show
BGM 8-bromo-2'-deoxyguanosine-5'-monophosphate
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 4NLK   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE, LYASE/DNA
Title : Structure of human DNA polymerase beta complexed with 8BrG in the template base-paired with incoming non-hydrolyzable CTP
Release Data : 2014-01-22
Compound :
mol_id molecule chains
1 5'-D(*CP*CP*GP*AP*CP*(BGM)P*TP*CP*GP*CP*AP*TP*CP*AP*GP*C)-3' T
other_details: template
mol_id molecule chains
2 5'-D(*GP*CP*TP*GP*AP*TP*GP*CP*GP*A)-3' P
other_details: up primer
mol_id molecule chains
3 5'-D(P*GP*TP*CP*GP*G)-3' D
other_details: down primer
mol_id molecule chains
4 DNA polymerase beta A
ec: 2.7.7.7, 4.2.99.-
Source :
mol_id organism_scientific
1
synthetic: yes
mol_id organism_scientific
2
synthetic: yes
mol_id organism_scientific
3
synthetic: yes
mol_id organism_scientific organism_common expression_system
4 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:562)
gene: POLB
Authors : Koag, M.-C., Min, K., Monzingo, A.F., Lee, S.
Keywords : DNA binding, helix-turn-helix motif, polymerase fold, nucleotidyl transferase, nucleus, TRANSFERASE, LYASE-DNA complex
Exp. method : X-RAY DIFFRACTION ( 2.494 Å )
Citation :

Structural basis for promutagenicity of 8-halogenated Guanine.

Koag, M.C.,Min, K.,Lee, S.
(2014)  J.Biol.Chem.  289 : 6289 - 6298

PubMed: 24425881
DOI: 10.1074/jbc.M113.537803

Reaction

Chain : A
UniProt : P06746 (DPOLB_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1) 2.7.7.7 -
-
a 5'-end 2'-deoxyribose-2'-deoxyribonucleotide-DNA = (2E,4S)- 4-hydroxypenten-2-al-5-phosphate + a 5'-end 5'-monophospho-2'- deoxyribonucleoside-DNA + H(+) - PubMed:9572863, PubMed:9614142
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2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'- deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3- dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho- 2'-deoxyribonucleoside-DNA + H(+) 4.2.99.18 PubMed:9614142
-