Brand  (β version)

PDB ID: 4NCS

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Ligands
Code Name Style Show Link
PO4 Phosphate ion PoSSuM
FSR (2s,3s,4r,5r,6r)-5-acetamido-2,3-bis(fluoranyl)-4-oxidanyl-6-[(1s,2s)-1,2,3-tris(oxidanyl)propyl]oxane-2-carboxylic acid PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 4NCS   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : Human sialidase 2 in complex with 2,3-difluorosialic acid (covalent intermediate)
Release Data : 2013-12-25
Compound :
mol_id molecule chains synonym
1 Sialidase-2 A Cytosolic sialidase, N-acetyl-alpha-neuraminidase 2
ec: 3.2.1.18
mutation: D46A,H168N
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:562)
gene: NEU2
expression_system_strain: BL21
expression_system_vector_type: PLASMID
expression_system_plasmid: PGEX-2T
Authors : Buchini, S., Gallat, F.-X., Greig, I.R., Kim, J.-H., Wakatsuki, S., Chavas, L.M.G., Withers, S.G.
Keywords : Sialidase, Human neuraminidase, fluoro-sialic acid, Hydrolase
Exp. method : X-RAY DIFFRACTION ( 2.201 Å )
Citation :

Tuning mechanism-based inactivators of neuraminidases: mechanistic and structural insights.

Buchini, S.,Gallat, F.X.,Greig, I.R.  et al.
(2014)  Angew.Chem.Int.Ed.Engl.  53 : 3382 - 3386

PubMed: 24591206
DOI: 10.1002/anie.201309675

Reaction

Chain : A
UniProt : Q9Y3R4 (NEUR2_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha- (2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates. 3.2.1.18 PubMed:14613940, PubMed:22228546
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a ganglioside GD1a + H2O = a ganglioside GM1 + N- acetylneuraminate - PubMed:14613940
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a ganglioside GM1 + H2O = a ganglioside GA1 + N- acetylneuraminate - PubMed:14613940
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a ganglioside GT1b + H2O = a ganglioside GD1b + N- acetylneuraminate - PubMed:14613940
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a ganglioside GD1b + H2O = a ganglioside GM1 + N- acetylneuraminate - PubMed:14613940
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a ganglioside GD3 + H2O = a ganglioside GM3 + N- acetylneuraminate - UniProtKB:Q9JMH3
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a ganglioside GM3 + H2O = a beta-D-galactosyl-(1->4)-beta-D- glucosyl-(1<->1)-ceramide + N-acetylneuraminate - PubMed:14613940, PubMed:22228546
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a ganglioside GM2 + H2O = a ganglioside GA2 + N- acetylneuraminate - UniProtKB:Q9JMH3
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a neolactoside IV(3)-alpha-NeuAc-nLc4Cer(d18:1(4E)) + H2O = a neolactoside nLc4Cer(d18:1(4E)) + N-acetylneuraminate - PubMed:14613940
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H2O + N-acetyl-alpha-neuraminosyl-(2->3)-beta-D-galactosyl- (1->4)-D-glucose = lactose + N-acetylneuraminate - PubMed:14613940, PubMed:22228546
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