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Ligands
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CZH C2-hydroperoxy-coelenterazine
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Glycosylation
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Code : 4MRX   PDBj   RCSB PDB   PDBe
Header : LUMINESCENT PROTEIN
Title : Crystal Structure of Y138F obelin mutant from Obelia longissima at 1.72 Angstrom resolution
Release Data : 2014-03-12
Compound :
mol_id molecule chains synonym
1 Obelin A OBL
mutation: S2A, Y138F
Source :
mol_id organism_scientific expression_system
1 Obelia longissima  (taxid:32570) Escherichia coli  (taxid:562)
expression_system_strain: Bl21 Gold
expression_system_vector_type: Plasmid
expression_system_plasmid: pOL-138F
Authors : Natashin, P.V., Ding, W., Eremeeva, E.V., Markova, S.V., Lee, J., Vysotski, E.S., Liu, Z.J.
Keywords : Luminescent Protein, calcium binding, EF-hand
Exp. method : X-RAY DIFFRACTION ( 1.718 Å )
Citation :

Structures of the Ca2+-regulated photoprotein obelin Y138F mutant before and after bioluminescence support the catalytic function of a water molecule in the reaction.

Natashin, P.V.,Ding, W.,Eremeeva, E.V.  et al.
(2014)  Acta Crystallogr.,Sect.D  70 : 720 - 732

PubMed: 24598741
DOI: 10.1107/S1399004713032434

Chain : A
UniProt : Q27709 (OBL_OBELO)
Reaction : -