Brand  (β version)

PDB ID: 4MQ9

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Ligands
Code Name Style Show Link
MB8 (2z)-2-methylbut-2-enoic acid PoSSuM
MG Magnesium ion PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
Code Name Show
0QZ D-isoserine
2RA 3-amino-D-alanine
2TL D-allothreonine
DSN D-serine
DVA D-valine
FGL 2-aminopropanedioic acid
R2T Beta,gamma-dihydroxyglutamine
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 4MQ9   PDBj   RCSB PDB   PDBe
Header : TRANSCRIPTION, TRANSFERASE/ANTIBIOTIC
Title : Crystal structure of Thermus thermophilus RNA polymerase holoenzyme in complex with GE23077
Release Data : 2014-05-07
Compound :
mol_id molecule chains synonym
1 DNA-directed RNA polymerase subunit alpha A,B RNAP subunit alpha, RNA polymerase subunit alpha, Transcriptase subunit alpha
ec: 2.7.7.6
fragment: RPOA
mol_id molecule chains synonym
2 DNA-directed RNA polymerase subunit beta C RNAP subunit beta, RNA polymerase subunit beta, Transcriptase subunit beta
ec: 2.7.7.6
fragment: RPOB
mol_id molecule chains synonym
3 DNA-directed RNA polymerase subunit beta' D RNAP subunit beta', RNA polymerase subunit beta', Transcriptase subunit beta'
ec: 2.7.7.6
fragment: RPOC
mol_id molecule chains synonym
4 DNA-directed RNA polymerase subunit omega E RNAP omega subunit, RNA polymerase omega subunit, Transcriptase subunit omega
ec: 2.7.7.6
fragment: RPOZ
mol_id molecule chains
5 RNA polymerase sigma factor F
ec: 2.7.7.6
fragment: RPOD
mol_id molecule chains
6 GE23077 I
Source :
mol_id organism_scientific
1 Thermus thermophilus  (taxid:300852)
strain: HB8
mol_id organism_scientific
2 Thermus thermophilus  (taxid:300852)
strain: HB8
mol_id organism_scientific
3 Thermus thermophilus  (taxid:300852)
strain: HB8
mol_id organism_scientific
4 Thermus thermophilus  (taxid:300852)
strain: HB8
mol_id organism_scientific expression_system
5 Thermus thermophilus  (taxid:300852) Escherichia coli  (taxid:562)
strain: HB8
gene: RPOD, TTHA0532
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: PET28A
mol_id organism_scientific
6 Actinomadura sp.  (taxid:1989)
Authors : Ho, M.X., Arnold, E., Ebright, R.H., Zhang, Y., Tuske, S.
Keywords : DNA-directed RNA polymerase, transcription, transferase-antibiotic complex
Exp. method : X-RAY DIFFRACTION ( 3.350 Å )
Citation :

GE23077 binds to the RNA polymerase 'i' and 'i+1' sites and prevents the binding of initiating nucleotides.

Zhang, Y.,Degen, D.,Ho, M.X.  et al.
(2014)  Elife  3 : e02450 - e02450

PubMed: 24755292

Reaction

Chain : A, B
UniProt : Q5SHR6 (RPOA_THET8)
Reaction: EC: Evidence:
Physiological Direction:
a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1) 2.7.7.6 -
-
Chain : C
UniProt : Q8RQE9 (RPOB_THET8)
Reaction: EC: Evidence:
Physiological Direction:
a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1) 2.7.7.6 HAMAP-Rule:MF_01321
-
Chain : D
UniProt : Q8RQE8 (RPOC_THET8)
Reaction: EC: Evidence:
Physiological Direction:
a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1) 2.7.7.6 HAMAP-Rule:MF_01322
-
Chain : E
UniProt : Q8RQE7 (RPOZ_THET8)
Reaction: EC: Evidence:
Physiological Direction:
a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1) 2.7.7.6 HAMAP-Rule:MF_00366
-
Chain : F
UniProt : Q5SKW1 (Q5SKW1_THET8)
Reaction : -