Brand  (β version)

PDB ID: 4MKK

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Ligands
Code Name Style Show Link
CL Chloride ion PoSSuM
K Potassium ion PoSSuM
NA Sodium ion PoSSuM
PGE Triethylene glycol PoSSuM
Non-standard Residues
Code Name Show
C1S 3-(prop-2-en-1-yldisulfanyl)-L-alanine
LLP (2s)-2-amino-6-[[3-hydroxy-2-methyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]hexanoic acid
Glycosylation
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Modification
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Structure summary

Code : 4MKK   PDBj   RCSB PDB   PDBe
Header : LYASE
Title : Crystal structure of C115A mutant L-methionine gamma-lyase from Citrobacter freundii modified by allicine
Release Data : 2014-11-12
Compound :
mol_id molecule chains
1 Methionine gamma-lyase A
ec: 4.4.1.11
mutation: C115A
Source :
mol_id organism_scientific expression_system
1 Citrobacter freundii  (taxid:546) Escherichia coli  (taxid:469008)
gene: megL
expression_system_strain: Bl21(DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: PET-C115AMGL
Authors : Revtovich, S.V., Nikulin, A.D., Morozova, E.A., Zakomirdina, L.N., Demidkina, T.V.
Keywords : pyridoxal-5'-phosphate, PLP-dependent enzyme, Aminotransferase class-V, lyase, allicine
Exp. method : X-RAY DIFFRACTION ( 1.45 Å )
Citation :

Alliin is a suicide substrate of Citrobacter freundii methionine gamma-lyase: structural bases of inactivation of the enzyme.

Morozova, E.A.,Revtovich, S.V.,Anufrieva, N.V.  et al.
(2014)  Acta Crystallogr.,Sect.D  70 : 3034 - 3042

PubMed: 25372692
DOI: 10.1107/S1399004714020938

Reaction

Chain : A
UniProt : Q84AR1 (Q84AR1_CITFR)
Reaction: EC: Evidence:
Physiological Direction:
H2O + L-methionine = 2-oxobutanoate + methanethiol + NH4(+) 4.4.1.11 ARBA:ARBA00000271
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