Brand  (β version)

PDB ID: 4KKX

Hetero Atom Contents

  The number of atoms exceeds 100,000.
  So, it can not be displayed here.

Select unit:

Select hetatm:   

close
information
centroid:
interaction residue:

Select chain:   Sequence  

Data format:   

Color scheme of protein:

Ligands
Code Name Style Show Link
AQ3 N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-3-[(2-hydroxyphenyl)amino]-D-alanine PoSSuM
EDO 1,2-ethanediol PoSSuM
F6F 2-{[4-(trifluoromethoxy)benzoyl]amino}ethyl dihydrogen phosphate PoSSuM
NA Sodium ion PoSSuM
PEG Di(hydroxyethyl)ether PoSSuM
PGE Triethylene glycol PoSSuM
Non-standard Residues
Code Name Show
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 4KKX   PDBj   RCSB PDB   PDBe
Header : LYASE/LYASE INHIBITOR
Title : Crystal structure of Tryptophan Synthase from Salmonella typhimurium with 2-aminophenol quinonoid in the beta site and the F6 inhibitor in the alpha site
Release Data : 2014-01-01
Compound :
mol_id molecule chains
1 Tryptophan synthase alpha chain A
ec: 4.2.1.20
mol_id molecule chains
2 Tryptophan synthase beta chain B
ec: 4.2.1.20
Source :
mol_id organism_scientific
1 Salmonella enterica subsp. enterica serovar Typhimurium  (taxid:90371)
gene: trpA
expression_system_strain: Escherichia coli
expression_system_cell_line: CB149
expression_system_vector_type: Derivative of pBR322
mol_id organism_scientific
2 Salmonella enterica subsp. enterica serovar Typhimurium  (taxid:90371)
gene: trpB
expression_system_strain: Escherichia coli
expression_system_cell_line: CB149
expression_system_vector_type: Derivative of pBR322
Authors : Hilario, E., Niks, D., Dunn, M.F., Mueller, L.J., Fan, L.
Keywords : Lyase, carbon-oxygen lyase, tryptophan biosynthesis, F6F, allosteric enzyme, 2-aminophenol quinonoid, biosynthesis, aromatic amino acid biosynthesis, pyridoxal phosphate, LYASE-LYASE INHIBITOR complex
Exp. method : X-RAY DIFFRACTION ( 1.7700 Å )
Citation :

Allostery and substrate channeling in the tryptophan synthase bienzyme complex: evidence for two subunit conformations and four quaternary states.

Niks, D.,Hilario, E.,Dierkers, A.  et al.
(2013)  Biochemistry  52 : 6396 - 6411

PubMed: 23952479
DOI: 10.1021/bi400795e

Reaction

Chain : A
UniProt : P00929 (TRPA_SALTY)
Reaction: EC: Evidence:
Physiological Direction:
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D- glyceraldehyde 3-phosphate + H2O + L-tryptophan 4.2.1.20 HAMAP-Rule:MF_00131
-
Chain : B
UniProt : P0A2K1 (TRPB_SALTY)
Reaction: EC: Evidence:
Physiological Direction:
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D- glyceraldehyde 3-phosphate + H2O + L-tryptophan 4.2.1.20 -
-