Het-PDB Navi2 (PDB: 4KAT)

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Ligands
Code	Name		Style	Show	Link
DU	2'-deoxyuridine-5'-monophosphate				PoSSuM
FDA	Dihydroflavine-adenine dinucleotide				PoSSuM

Non-standard Residues
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Glycosylation
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Download interaction data: 4KAT

Code :

4KAT PDBj RCSB PDB PDBe

Header :

TRANSFERASE

Title :

Crystal structure of FDTS from T. maritima mutant (R174K) with FAD and dUMP

Release Data :

2014-03-26

Compound :

mol_id	molecule	chains	synonym
1	Thymidylate synthase	A,B,C,D	TS, TSase
	ec: 2.1.1.148 fragment: TM0449 (unp residues 1-220) mutation: R174K

Source :

mol_id	organism_scientific	expression_system
1	Thermotoga maritima MSB8 (taxid:243274)	Escherichia coli (taxid:562)
	strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099 gene: thy1, thyX, tm0449, TM_0449 expression_system_vector_type: Plasmid expression_system_plasmid: pMH1

Authors :

Mathews, I.I.

Keywords :

thyX, FDTS, FAD, dUMP, Novel FDTS fold, Convertion of dUMP to dTMP using Tetrahydrofolate, TRANSFERASE

Exp. method :

X-RAY DIFFRACTION ( 2.14 Å )

Citation :

Flavin-Dependent Thymidylate Synthase as a Drug Target for Deadly Microbes: Mutational Study and a Strategy for Inhibitor Design.

Mathews, I.I.
(2013) J Bioterror Biodef Suppl 12 : 004 - 004

Chain :

A, B, C, D

UniProt :

Q9WYT0 (THYX_THEMA)

Reaction:	EC:	Evidence:
Reaction:	Physiological Direction:	Evidence:
dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADPH + H(+) = dTMP + (6S)-5,6,7,8-tetrahydrofolate + NADP(+)	2.1.1.148	PubMed:12791256, PubMed:19370033
	-	PubMed:12791256, PubMed:19370033
dUMP + formaldehyde + NADPH + H(+) = dTMP + NADP(+) + H2O	-	PubMed:34315871
dUMP + formaldehyde + NADPH + H(+) = dTMP + NADP(+) + H2O	-	PubMed:34315871
Cofactor:	Evidence:	Note:
FAD	ECO:0000269 \| PubMed:12791256 ECO:0000305 \| PubMed:12211025	Binds 4 FAD per tetramer. Each FAD binding site is formed by three monomers.

PDB ID: 4KAT