Brand  (β version)

PDB ID: 4KAS

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Ligands
Code Name Style Show Link
2PE Nonaethylene glycol PoSSuM
CL Chloride ion PoSSuM
DU 2'-deoxyuridine-5'-monophosphate PoSSuM
FAD Flavin-adenine dinucleotide PoSSuM
PO4 Phosphate ion PoSSuM
SO4 Sulfate ion PoSSuM
Non-standard Residues
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Structure summary

Code : 4KAS   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Crystal structure of FDTS from T. maritima mutant (H53D) with FAD and dUMP
Release Data : 2014-03-26
Compound :
mol_id molecule chains synonym
1 Thymidylate synthase ThyX A,B,C,D TS, TSase
ec: 2.1.1.148
fragment: TM0449 (unp residues 1-220)
mutation: H53D
Source :
mol_id organism_scientific expression_system
1 Thermotoga maritima  (taxid:243274) Escherichia coli  (taxid:562)
strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099
gene: thy1, thyX, tm0449, TM_0449
expression_system_vector_type: Plasmid
expression_system_plasmid: pMH1
Authors : Mathews, I.I.
Keywords : thyX, FDTS, FAD, dUMP, Novel FDTS fold, Convertion of dUMP to dTMP using Tetrahydrofolate, and NAD(P)Hc, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 1.85 Å )
Citation :

Flavin-Dependent Thymidylate Synthase as a Drug Target for Deadly Microbes: Mutational Study and a Strategy for Inhibitor Design.

Mathews, I.I.
(2013)  J Bioterror Biodef  Suppl 12 : 004 - 004

PubMed: 24563811
DOI: 10.4172/2157-2526.S12-004

Reaction

Chain : A, B, C, D
UniProt : Q9WYT0 (THYX_THEMA)
Reaction: EC: Evidence:
Physiological Direction:
dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADPH + H(+) = dTMP + (6S)-5,6,7,8-tetrahydrofolate + NADP(+) 2.1.1.148 PubMed:12791256, PubMed:19370033
-
dUMP + formaldehyde + NADPH + H(+) = dTMP + NADP(+) + H2O - PubMed:34315871
-
Cofactor: Evidence: Note:
FAD ECO:0000269 | PubMed:12791256
ECO:0000305 | PubMed:12211025
Binds 4 FAD per tetramer. Each FAD binding site is formed by three monomers.