Brand  (β version)

PDB ID: 4IZT

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Ligands
Code Name Style Show Link
ACM Acetamide PoSSuM
FTM 2-fluoroacetamide PoSSuM
Non-standard Residues
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Glycosylation
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Modification
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Structure summary

Code : 4IZT   PDBj   RCSB PDB   PDBe
Header : hydrolase/substrate
Title : The E41Q mutant of the amidase from Nesterenkonia sp. AN1 showing covalent addition of the acetamide moiety of fluoroacetamide at the active site cysteine
Release Data : 2014-02-12
Compound :
mol_id molecule chains
1 Amidase A
ec: 3.5.1.4
mutation: E41Q
Source :
mol_id organism_scientific expression_system
1 Nesterenkonia sp. 10004  (taxid:501897) Escherichia coli  (taxid:469008)
strain: AN1
gene: Nit2
expression_system_strain: BL21(DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pET28a
Authors : Kimani, S.W., Sewell, B.T.
Keywords : fluoroacetamide, acetamide, hydrolase-substrate complex
Exp. method : X-RAY DIFFRACTION ( 1.9200 Å )
Citation :

Covalent modifications of the active site cysteine occur as a result of mutating the glutamate of the catalytic triad in the amidase from Nesterenkonia sp.

Kimani, S.W.,Hunter, R.,Vlok, M.  et al.
To be Published 

Reaction

Chain : A
UniProt : D0VWZ1 (D0VWZ1_9MICC)
Reaction : -