Brand  (β version)

PDB ID: 4I50

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Ligands
Code Name Style Show Link
ACP Phosphomethylphosphonic acid adenylate ester PoSSuM
CA Calcium ion PoSSuM
CL Chloride ion PoSSuM
EP Epothilone a PoSSuM
GDP Guanosine-5'-diphosphate PoSSuM
GOL Glycerol PoSSuM
GTP Guanosine-5'-triphosphate PoSSuM
MES 2-(N-morpholino)-ethanesulfonic acid PoSSuM
MG Magnesium ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 4I50   PDBj   RCSB PDB   PDBe
Header : CELL CYCLE
Title : Crystal structure of tubulin-stathmin-TTL-Epothilone A complex
Release Data : 2013-01-23
Compound :
mol_id molecule chains synonym
1 Tubulin alpha-1B chain A,C Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain
mol_id molecule chains
2 Tubulin beta-2B chain B,D
mol_id molecule chains synonym
3 Stathmin-4 E Stathmin-like protein B3, RB3
mol_id molecule chains
4 Tubulin Tyrosine Ligase, TTL F
Source :
mol_id organism_scientific organism_common
1 Bos taurus  (taxid:9913) Bovine,cow,domestic cattle,domestic cow
organ: Brain
mol_id organism_scientific organism_common
2 Bos taurus  (taxid:9913) Bovine,cow,domestic cattle,domestic cow
organ: Brain
mol_id organism_scientific organism_common expression_system
3 Rattus norvegicus  (taxid:10116) Brown rat,rat,rats Escherichia coli  (taxid:562)
gene: Stmn4
expression_system_strain: NiCo21 (DE3)
expression_system_vector_type: pET3d
mol_id organism_scientific organism_common expression_system
4 Gallus gallus  (taxid:9031) Bantam,chickens Escherichia coli  (taxid:469008)
gene: TTL
expression_system_strain: BL21 (DE3)
expression_system_vector_type: NSKn1
expression_system_plasmid: PA23_007_ggTTL_KH6_2-378_NSKn1
Authors : Prota, A.E., Bargsten, K., Zurwerra, D., Field, J.J., Diaz, J.F., Altmann, K.H., Steinmetz, M.O.
Keywords : alpha-tubulin, beta-tubulin, ligase, GTPase, microtubule, stathmin, epothilone A, CELL CYCLE
Exp. method : X-RAY DIFFRACTION ( 2.300 Å )
Citation :

Molecular Mechanism of Action of Microtubule-Stabilizing Anticancer Agents.

Prota, A.E.,Bargsten, K.,Zurwerra, D.  et al.
(2013)  Science  339 : 587 - 590

PubMed: 23287720
DOI: 10.1126/science.1230582

Reaction

Chain : A, C
UniProt : P81947 (TBA1B_BOVIN)
Reaction: EC: Evidence:
Physiological Direction:
GTP + H2O = GDP + H(+) + phosphate - UniProtKB:P68363
left-to-right
Chain : B, D
UniProt : Q6B856 (TBB2B_BOVIN)
Reaction : -
Chain : E
UniProt : P63043 (STMN4_RAT)
Reaction : -
Chain : F
UniProt : E1BQ43
Reaction : -