Brand  (β version)

PDB ID: 4HDA

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Ligands
Code Name Style Show Link
ZN Zinc ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 4HDA   PDBj   RCSB PDB   PDBe
Header : HYDROLASE/HYDROLASE ACTIVATOR
Title : Crystal structure of human Sirt5 in complex with Fluor-de-Lys peptide and resveratrol
Release Data : 2012-12-05
Compound :
mol_id molecule chains synonym
1 NAD-dependent protein deacylase sirtuin-5, mitochondrial A,B Regulatory protein SIR2 homolog 5, SIR2-like protein 5
ec: 3.5.1.-
fragment: UNP residues 34-302
mol_id molecule chains
2 Fluor-de-Lys peptide F
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:469008)
gene: SIR2L5, SIRT5
expression_system_strain: BL21 (DE3) Rosetta2
expression_system_vector_type: plasmid
expression_system_plasmid: pET151/D-TOPO
mol_id organism_scientific
2
synthetic: yes
other_details: coumarin-modified tetra peptide
Authors : Gertz, M., Steegborn, C.
Keywords : NAD-dependent deacetylase, Sirtuin, mitochondrial, activator complex, resveratrol, HYDROLASE-HYDROLASE ACTIVATOR complex
Exp. method : X-RAY DIFFRACTION ( 2.6010 Å )
Citation :

A molecular mechanism for direct sirtuin activation by resveratrol.

Gertz, M.,Nguyen, G.T.,Fischer, F.  et al.
(2012)  Plos One  7 : e49761 - e49761

PubMed: 23185430
DOI: 10.1371/journal.pone.0049761

Reaction

Chain : A, B
UniProt : Q9NXA8 (SIR5_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
N(6)-malonyl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-malonyl- ADP-D-ribose + nicotinamide + L-lysyl-[protein] - HAMAP-Rule:MF_03160, PubMed:22076378
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N(6)-succinyl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O- succinyl-ADP-D-ribose + nicotinamide + L-lysyl-[protein] - HAMAP-Rule:MF_03160, PubMed:22076378, PubMed:29180469
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N(6)-glutaryl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O- glutaryl-ADP-D-ribose + nicotinamide + L-lysyl-[protein] - HAMAP-Rule:MF_03160, PubMed:24703693
-
Cofactor: Evidence: Note:
Zn(2+) ECO:0000255 | HAMAP-Rule:MF_03160
ECO:0000269 | PubMed:22076378
Binds 1 zinc ion per subunit.