Brand  (β version)

PDB ID: 4HD8

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Ligands
Code Name Style Show Link
IPA Isopropyl alcohol PoSSuM
PIT Piceatannol PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
Code Name Show
FDL N~6~-acetyl-N-(4-methyl-2-oxo-2h-chromen-7-yl)-L-lysinamide
Glycosylation
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Modification
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Structure summary

Code : 4HD8   PDBj   RCSB PDB   PDBe
Header : HYDROLASE/HYDROLASE INHIBITOR
Title : Crystal structure of human Sirt3 in complex with Fluor-de-Lys peptide and piceatannol
Release Data : 2012-12-05
Compound :
mol_id molecule chains synonym
1 NAD-dependent protein deacetylase sirtuin-3, mitochondrial A hSIRT3, Regulatory protein SIR2 homolog 3, SIR2-like protein 3
ec: 3.5.1.-
fragment: UNP residues 116-399
mol_id molecule chains
2 Fluor-de-Lys peptide F
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:469008)
gene: SIR2L3, SIRT3
expression_system_strain: BL21 (DE3) Rosetta2
expression_system_vector_type: plasmid
expression_system_plasmid: pVFT3S
mol_id organism_scientific
2
synthetic: yes
other_details: coumarin-modified tetra peptide
Authors : Nguyen, G.T.T., Gertz, M., Steegborn, C.
Keywords : NAD-dependent deacetylase, Sirtuin, mitochondrial, inhibitor complex, piceatannol, resveratrol-like compound, HYDROLASE-HYDROLASE INHIBITOR complex
Exp. method : X-RAY DIFFRACTION ( 2.3000 Å )
Citation :

A molecular mechanism for direct sirtuin activation by resveratrol.

Gertz, M.,Nguyen, G.T.,Fischer, F.  et al.
(2012)  Plos One  7 : e49761 - e49761

PubMed: 23185430
DOI: 10.1371/journal.pone.0049761

Reaction

Chain : A
UniProt : Q9NTG7 (SIR3_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
H2O + N(6)-acetyl-L-lysyl-[protein] + NAD(+) = 2''-O-acetyl- ADP-D-ribose + L-lysyl-[protein] + nicotinamide 2.3.1.286 PROSITE-ProRule:PRU00236, PubMed:12186850, PubMed:12374852, PubMed:16788062, PubMed:18680753, PubMed:18794531, PubMed:19535340, PubMed:24121500, PubMed:23283301
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