Brand  (β version)

PDB ID: 4H2B

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Ligands
Code Name Style Show Link
0XE 5,6-dihydroxy-4-oxo-2-phenyl-4h-chromen-7-yl beta-D-glucopyranosiduronic acid PoSSuM
CA Calcium ion PoSSuM
CL Chloride ion PoSSuM
ZN Zinc ion PoSSuM
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Structure summary

Code : 4H2B   PDBj   RCSB PDB   PDBe
Header : hydrolase/hydrolase inhibitor
Title : Human ecto-5'-nucleotidase (CD73): crystal form II (open) in complex with Baicalin
Release Data : 2012-11-28
Compound :
mol_id molecule chains synonym
1 5'-nucleotidase A 5'-NT, Ecto-5'-nucleotidase
ec: 3.1.3.5
fragment: unp residues 27-549
mutation: N53D, K145S, K147S, N311D, N333D, N403D, K478S
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:562)
gene: NT5, NT5E, NTE
expression_system_strain: Rosetta pLysS
expression_system_vector_type: plasmid
expression_system_plasmid: pET45b(+)
Authors : Zebisch, M., Pippel, J., Knapp, K., Straeter, N.
Keywords : dimer, hydrolase phosphatase nucleotidase, extracellular, hydrolase-hydrolase inhibitor complex
Exp. method : X-RAY DIFFRACTION ( 1.7 Å )
Citation :

Crystal Structure of the Human Ecto-5'-Nucleotidase (CD73): Insights into the Regulation of Purinergic Signaling.

Knapp, K.,Zebisch, M.,Pippel, J.  et al.
(2012)  Structure  20 : 2161 - 2173

PubMed: 23142347
DOI: 10.1016/j.str.2012.10.001

Reaction

Chain : A
UniProt : P21589 (5NTD_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate 3.1.3.5 PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084
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a 2'-deoxyribonucleoside 5'-phosphate + H2O = a 2'- deoxyribonucleoside + phosphate 3.1.3.89 PubMed:22997138, PubMed:34403084
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dTMP + H2O = phosphate + thymidine 3.1.3.35 PubMed:22997138, PubMed:34403084
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CMP + H2O = cytidine + phosphate 3.1.3.91 PubMed:22997138, PubMed:34403084
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H2O + IMP = inosine + phosphate 3.1.3.99 PubMed:22997138, PubMed:34403084
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AMP + H2O = adenosine + phosphate - PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084
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GMP + H2O = guanosine + phosphate - PubMed:22997138, PubMed:34403084
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H2O + UMP = phosphate + uridine - PubMed:22997138, PubMed:34403084
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dAMP + H2O = 2'-deoxyadenosine + phosphate - PubMed:22997138, PubMed:34403084
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dCMP + H2O = 2'-deoxycytidine + phosphate - PubMed:34403084
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