Brand  (β version)

PDB ID: 4H1Y

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Ligands
Code Name Style Show Link
0YQ 2,2'-[(2-{[2-({[(2s,3s,4r,5r)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2h)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]carbonyl}amino)ethyl]amino}-2-oxoethyl)imino]diacetic acid (non-preferred name) PoSSuM
CA Calcium ion PoSSuM
CL Chloride ion PoSSuM
GOL Glycerol PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
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Structure summary

Code : 4H1Y   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : Human ecto-5'-nucleotidase (CD73): crystal form II (open) in complex with PSB11552
Release Data : 2012-11-28
Compound :
mol_id molecule chains synonym
1 5'-nucleotidase P 5'-NT, Ecto-5'-nucleotidase
ec: 3.1.3.5
fragment: unp residues 27-549
mutation: N53D, N311D, N333D, N403D, K145S, K147S, K478S
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:562)
gene: NT5E, NT5, NTE
expression_system_strain: Rossetta (DE3) pLysS
expression_system_vector_type: Plasmid
expression_system_plasmid: pEt45b(+)
Authors : Pippel, J., Zebisch, M., Knapp, K., Straeter, N.
Keywords : dimeric, nucleotidase, hydrolase, AMP binding, Zinc binding, extracellular
Exp. method : X-RAY DIFFRACTION ( 1.58 Å )
Citation :

Crystal Structure of the Human Ecto-5'-Nucleotidase (CD73): Insights into the Regulation of Purinergic Signaling.

Knapp, K.,Zebisch, M.,Pippel, J.  et al.
(2012)  Structure  20 : 2161 - 2173

PubMed: 23142347
DOI: 10.1016/j.str.2012.10.001

Reaction

Chain : P
UniProt : P21589 (5NTD_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate 3.1.3.5 PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084
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a 2'-deoxyribonucleoside 5'-phosphate + H2O = a 2'- deoxyribonucleoside + phosphate 3.1.3.89 PubMed:22997138, PubMed:34403084
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dTMP + H2O = thymidine + phosphate 3.1.3.35 PubMed:22997138, PubMed:34403084
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CMP + H2O = cytidine + phosphate 3.1.3.91 PubMed:22997138, PubMed:34403084
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IMP + H2O = inosine + phosphate 3.1.3.99 PubMed:22997138, PubMed:34403084
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AMP + H2O = adenosine + phosphate - PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084
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GMP + H2O = guanosine + phosphate - PubMed:22997138, PubMed:34403084
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UMP + H2O = uridine + phosphate - PubMed:22997138, PubMed:34403084
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dAMP + H2O = 2'-deoxyadenosine + phosphate - PubMed:22997138, PubMed:34403084
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dCMP + H2O = 2'-deoxycytidine + phosphate - PubMed:34403084
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Cofactor: Evidence: Note:
Zn(2+) ECO:0000269 | PubMed:22997138
ECO:0000269 | PubMed:23142347
ECO:0000269 | PubMed:34403084
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