Brand  (β version)

PDB ID: 4GZ3

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Ligands
Code Name Style Show Link
UDP Uridine-5'-diphosphate PoSSuM
SO4 Sulfate ion PoSSuM
0YT 2-acetamido-2-deoxy-5-thio-beta-D-glucopyranose PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 4GZ3   PDBj   RCSB PDB   PDBe
Header : transferase/peptide
Title : Crystal structure of human O-GlcNAc Transferase with UDP and a thioglycopeptide
Release Data : 2012-10-31
Compound :
mol_id molecule chains synonym
1 UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit A,C O-GlcNAc transferase subunit p110, O-linked N-acetylglucosamine transferase 110 kDa subunit, OGT
ec: 2.4.1.255
mol_id molecule chains synonym
2 Casein kinase II subunit alpha B,D CK II alpha
ec: 2.7.11.1
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:562)
gene: OGT
mol_id organism_scientific organism_common
2 Homo sapiens  (taxid:9606) Human
synthetic: yes
other_details: synthetic peptide
Authors : Lazarus, M.B., Jiang, J., Gloster, T.M., Zandberg, W.F., Vocadlo, D.J., Walker, S.
Keywords : OGT, O-GlcNAc, GT-B, Glycosyltransferase, O-GlcNAcylation, transferase-peptide complex
Exp. method : X-RAY DIFFRACTION ( 1.900 Å )
Citation :

Structural snapshots of the reaction coordinate for O-GlcNAc transferase.

Lazarus, M.B.,Jiang, J.,Gloster, T.M.  et al.
(2012)  Nat.Chem.Biol.  8 : 966 - 968

PubMed: 23103939
DOI: 10.1038/nchembio.1109

Reaction

Chain : A, C
UniProt : O15294 (OGT1_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
L-seryl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N- acetyl-beta-D-glucosaminyl)-L-seryl-[protein] + H(+) + UDP 2.4.1.255 PubMed:15361863, PubMed:21240259, PubMed:21285374, PubMed:23103939, PubMed:24563466, PubMed:27713473, PubMed:30699359, PubMed:34074792, PubMed:26369908, PubMed:26678539
left-to-right PubMed:24563466, PubMed:27713473, PubMed:30699359, PubMed:34074792
L-threonyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O- (N-acetyl-beta-D-glucosaminyl)-L-threonyl-[protein] + H(+) + UDP 2.4.1.255 PubMed:15361863, PubMed:21240259, PubMed:21285374, PubMed:24563466, PubMed:37541260, PubMed:26678539
left-to-right PubMed:24563466, PubMed:27713473, PubMed:37541260
[Isoform 4]
L-seryl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N- acetyl-beta-D-glucosaminyl)-L-seryl-[protein] + H(+) + UDP 		2.4.1.255 PubMed:31527085
left-to-right
[Isoform 4]
L-threonyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O- (N-acetyl-beta-D-glucosaminyl)-L-threonyl-[protein] + H(+) + UDP 		2.4.1.255 PubMed:31527085
left-to-right
Chain : B, D
UniProt : P68400 (CSK21_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl- [protein] 2.7.11.1 PubMed:20545769, PubMed:20625391, PubMed:21482717, PubMed:22184066, PubMed:23123191, PubMed:30699359, PubMed:30898438, PubMed:31439799
left-to-right PubMed:20545769, PubMed:21482717, PubMed:23123191, PubMed:30699359, PubMed:30898438, PubMed:31439799, PubMed:35597237
ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L- threonyl-[protein] 2.7.11.1 PubMed:20625391, PubMed:22325354, PubMed:31439799
left-to-right PubMed:31439799