Brand  (β version)

PDB ID: 4GD3

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Ligands
Code Name Style Show Link
SF4 Iron/sulfur cluster PoSSuM
F4S Fe4-s3 cluster PoSSuM
CL Chloride ion PoSSuM
FCO Carbonmonoxide-(dicyano) iron PoSSuM
NI Nickel (II) ion PoSSuM
MG Magnesium ion PoSSuM
HEM Protoporphyrin ix containing Fe PoSSuM
LMT Dodecyl-beta-D-maltoside PoSSuM
Non-standard Residues
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Structure summary

Code : 4GD3   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE/ELECTRON TRANSPORT
Title : Structure of E. coli hydrogenase-1 in complex with cytochrome B
Release Data : 2013-01-02
Compound :
mol_id molecule chains synonym
1 Hydrogenase-1 small chain S,T,Q,R HYD1, Membrane-bound hydrogenase 1 small subunit, NiFe hydrogenase
ec: 1.12.99.6
mutation: P287C
mol_id molecule chains synonym
2 Hydrogenase-1 large chain L,M,J,K HYD1, Membrane-bound hydrogenase 1 large subunit, NiFe hydrogenase
ec: 1.12.99.6
mol_id molecule chains
3 Ni/Fe-hydrogenase 1 B-type cytochrome subunit A,B
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:83333) Escherichia coli  (taxid:562)
strain: K12
gene: b0972, hyaA, JW0954
expression_system_strain: FTH004
mol_id organism_scientific expression_system
2 Escherichia coli  (taxid:83333) Escherichia coli  (taxid:562)
strain: K12
gene: b0973, hyaB, JW0955
expression_system_strain: FTH004
mol_id organism_scientific expression_system
3 Escherichia coli  (taxid:83333) Escherichia coli  (taxid:562)
strain: K12
gene: b0974, hyaC, JW0956
expression_system_strain: FTH004
Authors : Volbeda, A., Fontecilla-Camps, J.C., Darnault, C.
Keywords : O2-tolerant H2:quinone oxidoreductase, membrane-bound, Ni-Fe-hydrogenase-cytochrome B complex, OXIDOREDUCTASE-ELECTRON TRANSPORT complex
Exp. method : X-RAY DIFFRACTION ( 3.3000 Å )
Citation :

Crystal Structure of the O(2)-Tolerant Membrane-Bound Hydrogenase 1 from Escherichia coli in Complex with Its Cognate Cytochrome b.

Volbeda, A.,Darnault, C.,Parkin, A.  et al.
(2013)  Structure  21 : 184 - 190

PubMed: 23260654
DOI: 10.1016/j.str.2012.11.010

X-ray crystallographic and computational studies of the O2-tolerant [NiFe]-hydrogenase 1 from Escherichia coli.

Volbeda, A.,Amara, P.,Darnault, C.  et al.
(2012)  Proc.Natl.Acad.Sci.USA  109 : 5305 - 5310

PubMed: 22431599
DOI: 10.1073/pnas.1119806109

Reaction

Chain : S, T, Q, R
UniProt : P69739 (MBHS_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
A + H2 = AH2 1.12.99.6 -
-
Chain : L, M, J, K
UniProt : P0ACD8 (MBHL_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
A + H2 = AH2 1.12.99.6 -
-
Chain : A, B
UniProt : P0AAM1 (CYBH_ECOLI)
Reaction : -