Brand  (β version)

PDB ID: 4F5P

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Ligands
Code Name Style Show Link
F2A 2'-deoxy-5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]methyl}phosphoryl]adenosine PoSSuM
MG Magnesium ion PoSSuM
NA Sodium ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 4F5P   PDBj   RCSB PDB   PDBe
Header : Transferase, lyase/DNA
Title : Open ternary mismatch complex of R283K DNA polymerase beta with a dATP analog
Release Data : 2012-12-12
Compound :
mol_id molecule chains
1 DNA polymerase beta A
ec: 2.7.7.7, 4.2.99.-
mutation: R283K
mol_id molecule chains
2 DNA (5'-D(*CP*CP*GP*AP*CP*GP*GP*CP*GP*CP*AP*TP*CP*AP*GP*C)-3') T
mol_id molecule chains
3 DNA (5'-D(*GP*CP*TP*GP*AP*TP*GP*CP*GP*C)-3') P
mol_id molecule chains
4 DNA (5'-D(P*GP*TP*CP*GP*G)-3') D
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:562)
gene: POLB
expression_system_strain: Tap56
expression_system_vector_type: Plasmid
expression_system_plasmid: pWL11
mol_id organism_scientific
2 Synthetic construct  (taxid:32630)
synthetic: yes
other_details: polydeoxyribonucleotide from IDT
mol_id organism_scientific
3 Synthetic construct  (taxid:32630)
synthetic: yes
other_details: polydeoxyribonucleotide from IDT
mol_id organism_scientific
4 Synthetic construct  (taxid:32630)
synthetic: yes
other_details: polydeoxyribonucleotide from IDT
Authors : Freudenthal, B.D., Beard, W.A., Wilson, S.H.
Keywords : Transferase, lyase/DNA, lyase-DNA complex
Exp. method : X-RAY DIFFRACTION ( 1.85 Å )
Citation :

Structures of dNTP Intermediate States during DNA Polymerase Active Site Assembly.

Freudenthal, B.D.,Beard, W.A.,Wilson, S.H.
(2012)  Structure  20 : 1829 - 1837

PubMed: 22959623
DOI: 10.1016/j.str.2012.08.008

Reaction

Chain : A
UniProt : P06746 (DPOLB_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate 2.7.7.7 -
-
a 5'-end 2'-deoxyribose-2'-deoxyribonucleotide-DNA = (2E,4S)- 4-hydroxypenten-2-al-5-phosphate + a 5'-end 5'-phospho-2'- deoxyribonucleoside-DNA + H(+) - PubMed:9572863, PubMed:9614142
-
2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'- deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3- dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'- deoxyribonucleoside-DNA + H(+) 4.2.99.18 PubMed:9614142
-
Cofactor: Evidence: Note:
Mg(2+) ECO:0000269 | PubMed:9287163
Binds 2 magnesium ions per subunit.